Lamin A buffers CK2 kinase activity to modulate aging in a progeria mouse model

被引：28

作者：

Ao, Ying ^{[1
,4
]}

Zhang, Jie ^{[1
]}

Liu, Zuojun ^{[1
]}

Qian, Minxian ^{[1
]}

Li, Yao ^{[2
]}

Wu, Zhuping ^{[1
]}

Sun, Pengfei ^{[1
]}

Wu, Jie ^{[1
]}

Bei, Weixin ^{[1
]}

Wen, Junqu ^{[1
]}

Wu, Xuli ^{[2
]}

Li, Feng ^{[4
]}

Zhou, Zhongjun ^{[3
]}

Zhu, Wei-Guo ^{[1
]}

Liu, Baohua ^{[1
]}

Wang, Zimei ^{[1
]}

机构：

[1] Hlth Sci Ctr, Guangdong Key Lab Genome Stabil & Human Dis Preve, Carson Int Canc Ctr, Dept Biochem & Mol Biol,Sch Basic Med Sci, Shenzhen 518060, Peoples R China

[2] Shenzhen Univ, Sch Publ Hlth, Hlth Sci Ctr, Shenzhen 518060, Peoples R China

[3] Univ Hong Kong, Li Ka Shing Fac Med, Sch Biomed Sci, 21 Sassoon Rd, Hong Kong, Peoples R China

[4] Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430071, Hubei, Peoples R China

来源：

SCIENCE ADVANCES | 2019年 / 5卷 / 03期

基金：

中国国家自然科学基金;

关键词：

PROTEIN-KINASE; NUCLEAR-MATRIX; AMELIORATES DISEASE; BETA-SUBUNIT; INHIBITOR; PHOSPHORYLATES; SENESCENCE; EXPRESSION; MECHANISM; AUTOPHAGY;

D O I：

10.1126/sciadv.aav5078

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Defective nuclear lamina protein lamin A is associated with premature aging. Casein kinase 2 (CK2) binds the nuclear lamina, and inhibiting CK2 activity induces cellular senescence in cancer cells. Thus, it is feasible that lamin A and CK2 may cooperate in the aging process. Nuclear CK2 localization relies on lamin A and the lamin A carboxyl terminus physically interacts with the CK2 alpha catalytic core and inhibits its kinase activity. Loss of lamin A in Lmna-knockout mouse embryonic fibroblasts (MEFs) confers increased CK2 activity. Conversely, prelamin A that accumulates in Zmpste24-deficent MEFs exhibits a high CK2 alpha binding affinity and concomitantly reduces CK2 kinase activity. Permidine treatment activates CK2 by releasing the interaction between lamin A and CK2, promoting DNA damage repair and ameliorating progeroid features. These data reveal a previously unidentified function for nuclear lamin A and highlight an essential role for CK2 in regulating senescence and aging.

引用

页数：12

共 42 条

[1] HP1-β mobilization promotes chromatin changes that initiate the DNA damage response [J].

Ayoub, Nabieh ;

Jeyasekharan, Anand D. ;

Bernal, Juan A. ;

Venkitaraman, Ashok R. .

NATURE, 2008, 453 (7195) :682-U14

[2] The replacement of ATP by the competitive inhibitor emodin induces conformational modifications in the catalytic site of protein kinase CK2 [J].

Battistutta, R ;

Sarno, S ;

De Moliner, E ;

Papinutto, E ;

Zanotti, G ;

Pinna, LA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (38) :29618-29622

[3]

BOLDYREFF B, 1994, CELL MOL BIOL RES, V40, P391

[4]

BROERS JLV, 1993, AM J PATHOL, V143, P211

[5] Effects of p21Waf1/Cip1/Sdi1 on cellular gene expression:: Implications for carcinogenesis, senescence, and age-related diseases [J].

Chang, BD ;

Watanabe, K ;

Broude, EV ;

Fang, J ;

Poole, JC ;

Kalinichenko, TV ;

Roninson, IB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (08) :4291-4296

[6] Prelamin A causes progeria through cell-extrinsic mechanisms and prevents cancer invasion [J].

de la Rosa, Jorge ;

Freije, Jose M. P. ;

Cabanillas, Ruben ;

Osorio, Fernando G. ;

Fraga, Mario F. ;

Soledad Fernandez-Garcia, M. ;

Rad, Roland ;

Fanjul, Victor ;

Ugalde, Alejandro P. ;

Liang, Qi ;

Prosser, Haydn M. ;

Bradley, Allan ;

Cadinanos, Juan ;

Lopez-Otin, Carlos .

NATURE COMMUNICATIONS, 2013, 4

[7] Nuclear lamins: major factors in the structural organization and function of the nucleus and chromatin [J].

Dechat, Thomas ;

Pfleghaar, Katrin ;

Sengupta, Kaushik ;

Shimi, Takeshi ;

Shumaker, Dale K. ;

Solimando, Liliana ;

Goldman, Robert D. .

GENES & DEVELOPMENT, 2008, 22 (07) :832-853

[8] Protein kinase CK2 phosphorylates and upregulates Akt/PKB [J].

Di Maira, G ;

Salvi, M ;

Arrigoni, G ;

Marin, O ;

Sarno, S ;

Brustolon, F ;

Pinna, LA ;

Ruzzene, M .

CELL DEATH AND DIFFERENTIATION, 2005, 12 (06) :668-677

[9] Cardioprotection and lifespan extension by the natural polyamine spermidine [J].

Eisenberg, Tobias ;

Abdellatif, Mahmoud ;

Schroeder, Sabrina ;

Primessnig, Uwe ;

Stekovic, Slaven ;

Pendl, Tobias ;

Harger, Alexandra ;

Schipke, Julia ;

Zimmermann, Andreas ;

Schmidt, Albrecht ;

Tong, Mingming ;

Ruckenstuhl, Christoph ;

Dammbrueck, Christopher ;

Gross, Angelina S. ;

Herbst, Viktoria ;

Magnes, Christoph ;

Trausinger, Gert ;

Narath, Sophie ;

Meinitzer, Andreas ;

Hu, Zehan ;

Kirsch, Alexander ;

Eller, Kathrin ;

Carmona-Gutierrez, Didac ;

Buettner, Sabrina ;

Pietrocola, Federico ;

Knittelfelder, Oskar ;

Schrepfer, Emilie ;

Rockenfeller, Patrick ;

Simonini, Corinna ;

Rahn, Alexandros ;

Horsch, Marion ;

Moreth, Kristin ;

Beckers, Johannes ;

Fuchs, Helmut ;

Gailus-Durner, Valerie ;

Neff, Frauke ;

Janik, Dirk ;

Rathkolb, Birgit ;

Rozman, Jan ;

de Angelis, Martin Hrabe ;

Moustafa, Tarek ;

Haemmerle, Guenter ;

Mayr, Manuel ;

Willeit, Peter ;

von Frieling-Salewsky, Marion ;

Pieske, Burkert ;

Scorrano, Luca ;

Pieber, Thomas ;

Pechlaner, Raimund ;

Willeit, Johann .

NATURE MEDICINE, 2016, 22 (12) :1428-+

[10] Induction of autophagy by spermidine promotes longevity [J].

Eisenberg, Tobias ;

Knauer, Heide ;

Schauer, Alexandra ;

Buettner, Sabrina ;

Ruckenstuhl, Christoph ;

Carmona-Gutierrez, Didac ;

Ring, Julia ;

Schroeder, Sabrina ;

Magnes, Christoph ;

Antonacci, Lucia ;

Fussi, Heike ;

Deszcz, Luiza ;

Hartl, Regina ;

Schraml, Elisabeth ;

Criollo, Alfredo ;

Megalou, Evgenia ;

Weiskopf, Daniela ;

Laun, Peter ;

Heeren, Gino ;

Breitenbach, Michael ;

Grubeck-Loebenstein, Beatrix ;

Herker, Eva ;

Fahrenkrog, Birthe ;

Froehlich, Kai-Uwe ;

Sinner, Frank ;

Tavernarakis, Nektarios ;

Minois, Nadege ;

Kroemer, Guido ;

Madeo, Frank .

NATURE CELL BIOLOGY, 2009, 11 (11) :1305-U102

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