AMPHIPATHIC ALPHA-HELIX;
PROTEIN VIPERIN;
HUMAN CYTOMEGALOVIRUS;
REPLICATION;
INTERFERON;
EXPRESSION;
IDENTIFICATION;
MACROPHAGES;
SITES;
CELLS;
D O I:
10.1371/journal.pntd.0002178
中图分类号:
R51 [传染病];
学科分类号:
100401 ;
摘要:
The host protein viperin is an interferon stimulated gene (ISG) that is up-regulated during a number of viral infections. In this study we have shown that dengue virus type-2 (DENV-2) infection significantly induced viperin, co-incident with production of viral RNA and via a mechanism requiring retinoic acid-inducible gene I (RIG-I). Viperin did not inhibit DENV-2 entry but DENV-2 RNA and infectious virus release was inhibited in viperin expressing cells. Conversely, DENV-2 replicated to higher tires earlier in viperin shRNA expressing cells. The anti-DENV effect of viperin was mediated by residues within the C-terminal 17 amino acids of viperin and did not require the N-terminal residues, including the helix domain, leucine zipper and S-adenosylmethionine (SAM) motifs known to be involved in viperin intracellular membrane association. Viperin showed co-localisation with lipid droplet markers, and was co-localised and interacted with DENV-2 capsid (CA), NS3 and viral RNA. The ability of viperin to interact with DENV-2 NS3 was associated with its anti-viral activity, while co-localisation of viperin with lipid droplets was not. Thus, DENV-2 infection induces viperin which has anti-viral properties residing in the C-terminal region of the protein that act to restrict early DENV-2 RNA production/accumulation, potentially via interaction of viperin with DENV-2 NS3 and replication complexes. These anti-DENV-2 actions of viperin show both contrasts and similarities with other described anti-viral mechanisms of viperin action and highlight the diverse nature of this unique antiviral host protein.
机构:
Univ Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, PortugalUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Carvalho, Filomena A.
Carneiro, Fabiana A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Rio de Janeiro, Inst Bioquim Med, Rio De Janeiro, Brazil
Univ Fed Rio de Janeiro, Duque De Caxias, BrazilUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Carneiro, Fabiana A.
Martins, Ivo C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, PortugalUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Martins, Ivo C.
Assuncao-Miranda, Iranaia
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo Goes, Rio De Janeiro, BrazilUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Assuncao-Miranda, Iranaia
Faustino, Andre F.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, PortugalUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Faustino, Andre F.
Pereira, Renata M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Rio De Janeiro, BrazilUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Pereira, Renata M.
Bozza, Patricia T.
论文数: 0引用数: 0
h-index: 0
机构:
Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Rio De Janeiro, BrazilUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Bozza, Patricia T.
Castanho, Miguel A. R. B.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, PortugalUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Castanho, Miguel A. R. B.
Mohana-Borges, Ronaldo
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Rio De Janeiro, BrazilUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Mohana-Borges, Ronaldo
Da Poian, Andrea T.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Rio de Janeiro, Inst Bioquim Med, Rio De Janeiro, BrazilUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Da Poian, Andrea T.
Santos, Nuno C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, PortugalUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
机构:
Yale Univ, Sch Med, Howard Hughes Med Inst, Immunobiol Sect, New Haven, CT 06520 USAYale Univ, Sch Med, Howard Hughes Med Inst, Immunobiol Sect, New Haven, CT 06520 USA
Chin, KC
Cresswell, P
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Sch Med, Howard Hughes Med Inst, Immunobiol Sect, New Haven, CT 06520 USAYale Univ, Sch Med, Howard Hughes Med Inst, Immunobiol Sect, New Haven, CT 06520 USA
机构:
SA Pathol, Ctr Canc Biol, Adelaide, SA, Australia
Univ Adelaide, Sch Mol & Biomed Sci, Adelaide, SA, AustraliaSA Pathol, Ctr Canc Biol, Adelaide, SA, Australia
Eyre, Nicholas S.
Drummer, Heidi E.
论文数: 0引用数: 0
h-index: 0
机构:
Burnet Inst, Melbourne, Vic, Australia
Univ Melbourne, Dept Microbiol & Immunol, Parkville, Vic 3052, Australia
Monash Univ, Dept Microbiol, Clayton, Vic 3168, AustraliaSA Pathol, Ctr Canc Biol, Adelaide, SA, Australia
Drummer, Heidi E.
Beard, Michael R.
论文数: 0引用数: 0
h-index: 0
机构:
SA Pathol, Ctr Canc Biol, Adelaide, SA, Australia
Univ Adelaide, Sch Mol & Biomed Sci, Adelaide, SA, AustraliaSA Pathol, Ctr Canc Biol, Adelaide, SA, Australia
机构:
Univ Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, PortugalUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Carvalho, Filomena A.
Carneiro, Fabiana A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Rio de Janeiro, Inst Bioquim Med, Rio De Janeiro, Brazil
Univ Fed Rio de Janeiro, Duque De Caxias, BrazilUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Carneiro, Fabiana A.
Martins, Ivo C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, PortugalUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Martins, Ivo C.
Assuncao-Miranda, Iranaia
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo Goes, Rio De Janeiro, BrazilUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Assuncao-Miranda, Iranaia
Faustino, Andre F.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, PortugalUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Faustino, Andre F.
Pereira, Renata M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Rio De Janeiro, BrazilUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Pereira, Renata M.
Bozza, Patricia T.
论文数: 0引用数: 0
h-index: 0
机构:
Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Rio De Janeiro, BrazilUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Bozza, Patricia T.
Castanho, Miguel A. R. B.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, PortugalUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Castanho, Miguel A. R. B.
Mohana-Borges, Ronaldo
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Rio De Janeiro, BrazilUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Mohana-Borges, Ronaldo
Da Poian, Andrea T.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Rio de Janeiro, Inst Bioquim Med, Rio De Janeiro, BrazilUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
Da Poian, Andrea T.
Santos, Nuno C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, PortugalUniv Lisbon, Inst Mol Med, Fac Med, P-1200 Lisbon, Portugal
机构:
Yale Univ, Sch Med, Howard Hughes Med Inst, Immunobiol Sect, New Haven, CT 06520 USAYale Univ, Sch Med, Howard Hughes Med Inst, Immunobiol Sect, New Haven, CT 06520 USA
Chin, KC
Cresswell, P
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Sch Med, Howard Hughes Med Inst, Immunobiol Sect, New Haven, CT 06520 USAYale Univ, Sch Med, Howard Hughes Med Inst, Immunobiol Sect, New Haven, CT 06520 USA
机构:
SA Pathol, Ctr Canc Biol, Adelaide, SA, Australia
Univ Adelaide, Sch Mol & Biomed Sci, Adelaide, SA, AustraliaSA Pathol, Ctr Canc Biol, Adelaide, SA, Australia
Eyre, Nicholas S.
Drummer, Heidi E.
论文数: 0引用数: 0
h-index: 0
机构:
Burnet Inst, Melbourne, Vic, Australia
Univ Melbourne, Dept Microbiol & Immunol, Parkville, Vic 3052, Australia
Monash Univ, Dept Microbiol, Clayton, Vic 3168, AustraliaSA Pathol, Ctr Canc Biol, Adelaide, SA, Australia
Drummer, Heidi E.
Beard, Michael R.
论文数: 0引用数: 0
h-index: 0
机构:
SA Pathol, Ctr Canc Biol, Adelaide, SA, Australia
Univ Adelaide, Sch Mol & Biomed Sci, Adelaide, SA, AustraliaSA Pathol, Ctr Canc Biol, Adelaide, SA, Australia