The lac Repressor Displays Facilitated Diffusion in Living Cells

被引：314

作者：

Hammar, Petter ^{[1
]}

Leroy, Prune ^{[1
]}

Mahmutovic, Anel ^{[1
]}

Marklund, Erik G. ^{[1
]}

Berg, Otto G. ^{[1
]}

Elf, Johan ^{[1
]}

机构：

[1] Uppsala Univ, Dept Cell & Mol Biol, Sci Life Lab, Uppsala, Sweden

来源：

SCIENCE | 2012年 / 336卷 / 6088期

基金：

瑞典研究理事会; 欧洲研究理事会;

关键词：

ESCHERICHIA-COLI-LAC; PROTEIN TRANSLOCATION; OPERATOR INTERACTION; DRIVEN MECHANISMS; TARGET LOCATION; NUCLEIC-ACIDS; IN-VIVO; DNA; SEQUENCE; KINETICS;

D O I：

10.1126/science.1221648

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Transcription factors (TFs) are proteins that regulate the expression of genes by binding sequence-specific sites on the chromosome. It has been proposed that to find these sites fast and accurately, TFs combine one-dimensional (1D) sliding on DNA with 3D diffusion in the cytoplasm. This facilitated diffusion mechanism has been demonstrated in vitro, but it has not been shown experimentally to be exploited in living cells. We have developed a single-molecule assay that allows us to investigate the sliding process in living bacteria. Here we show that the lac repressor slides 45 T 10 base pairs on chromosomal DNA and that sliding can be obstructed by other DNA-bound proteins near the operator. Furthermore, the repressor frequently (>90%) slides over its natural lacO(1) operator several times before binding. This suggests a trade-off between rapid search on nonspecific sequences and fast binding at the specific sequence.

引用

页码：1595 / 1598

页数：4

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