Engineering genes for predictable protein expression

被引：122

作者：

Gustafsson, Claes ^{[1
]}

Minshull, Jeremy ^{[1
]}

Govindarajan, Sridhar ^{[1
]}

Ness, Jon ^{[1
]}

Villalobos, Alan ^{[1
]}

Welch, Mark ^{[1
]}

机构：

[1] DNA2 0 Inc, Menlo Pk, CA 94025 USA

来源：

PROTEIN EXPRESSION AND PURIFICATION | 2012年 / 83卷 / 01期

基金：

美国国家科学基金会;

关键词：

Heterologous protein expression; Bioengineering; Synthetic biology; Gene optimization; Codon usage; RNA SECONDARY STRUCTURE; CODON ADAPTATION INDEX; HIGH-LEVEL EXPRESSION; ESCHERICHIA-COLI; RECOMBINANT PROTEINS; ARGININE CODONS; TARGET GENE; USAGE BIAS; IN-VIVO; TRANSLATION;

D O I：

10.1016/j.pep.2012.02.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The DNA sequence used to encode a polypeptide can have dramatic effects on its expression. Lack of readily available tools has until recently inhibited meaningful experimental investigation of this phenomenon. Advances in synthetic biology and the application of modern engineering approaches now provide the tools for systematic analysis of the sequence variables affecting heterologous expression of recombinant proteins. We here discuss how these new tools are being applied and how they circumvent the constraints of previous approaches, highlighting some of the surprising and promising results emerging from the developing field of gene engineering. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：37 / 46

页数：10

共 88 条

[1] Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli [J].