Short Report: Comparison of Performance of Serum and Plasma in Panbio Dengue and Japanese Encephalitis Virus Enzyme-Linked Immunosorbent Assays

被引：9

作者：

Blacksell, Stuart D. ^{[1
,2
,3
]}

Lee, Sue J. ^{[1
,4
]}

Chanthongthip, Anisone

Taojaikong, Thaksinaporn

Thongpaseuth, Soulignasack ^{[3
]}

Huebscher, Tanja ^{[5
]}

Newton, Paul N. ^{[3
,4
]}

机构：

[1] Mahidol Univ, Fac Trop Med, Mahidol Oxford Trop Med Res Unit, Bangkok 10400, Thailand

[2] Univ Oxford, Ctr Clin Vaccinol & Trop Med, Churchill Hosp, Oxford OX3 7LJ, England

[3] Mahosot Hosp, Lao Oxford Mahosot Hosp Wellcome Trust Res Unit, Microbiol Lab, Viangchan, Laos

[4] Univ Oxford, Churchill Hosp, Ctr Trop Med, Oxford OX3 7LJ, England

[5] Hohmad Clin, Thun, Switzerland

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2012年 / 87卷 / 03期

基金：

英国惠康基金;

关键词：

INFECTION; DIAGNOSIS;

D O I：

10.4269/ajtmh.2012.12-0043

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

We examined the comparative performance of serum and plasma (in dipotassium EDTA) in Panbio Dengue enzyme-linked immunosorbent assays (ELISAs) for detection of non-structural protein 1 (NS1), IgM, and IgG, and a dengue/Japanese encephalitis virus (JEV) combination IgM ELISA in a prospective series of 201 patients with suspected dengue in Laos. Paired comparisons of medians from serum and plasma samples were not significantly different for Dengue IgM, and NS1 which had the highest number of discordant pairs (both 2%; P = 0.13 and P = 0.25, respectively). Comparison of qualitative final diagnostic interpretations for serum and plasma samples were not significantly different: only 1.5% (3 of 201 for Dengue/JEV IgM and Dengue IgG) and 2.0% (4 of 201; IgM and NS1) showed discordant pairs. These results demonstrate that plasma containing EDTA is suitable for use in these ELISAs.

引用

页码：573 / 575

页数：3

共 12 条

[1]

[Anonymous], 2019, Dengue Guidelines for Diagnosis, Treatment, Prevention and Control

[2] Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos [J].