A guide for using NIH Image J for single slice cross-sectional area and composition analysis of the thigh from computed tomography

被引:36
作者
Long, Douglas E. [1 ,2 ]
Tezanos, Alejandro G. Villasante [3 ]
Wise, James N. [4 ]
Kern, Philip A. [5 ,6 ]
Bamman, Marcas M. [7 ,8 ]
Peterson, Charlotte A. [1 ,2 ]
Dennis, Richard A. [9 ,10 ]
机构
[1] Univ Kentucky, Coll Hlth Sci, Lexington, KY 40506 USA
[2] Univ Kentucky, Ctr Muscle Biol, Lexington, KY 40506 USA
[3] Univ Kentucky, Dept Stat, Coll Arts & Sci, Lexington, KY USA
[4] Univ Arkansas Med Sci, Dept Radiol, Coll Med, Little Rock, AR 72205 USA
[5] Univ Kentucky, Dept Internal Med, Div Endocrinol, Lexington, KY USA
[6] Univ Kentucky, Barnstable Brown Diabet & Obes Ctr, Lexington, KY USA
[7] Univ Alabama Birmingham, Ctr Exercise Med, Birmingham, AL USA
[8] Univ Alabama Birmingham, Dept Cell Dev & Integrat Biol, Birmingham, AL USA
[9] Cent Arkansas Vet Healthcare Syst, Geriatr Res Educ & Clin Ctr, North Little Rock, AR USA
[10] Univ Arkansas Med Sci, Donald W Reynolds Dept Geriatr, Little Rock, AR 72205 USA
基金
美国国家卫生研究院;
关键词
MUSCLE RADIATION ATTENUATION; SKELETAL-MUSCLE; PHYSICAL FUNCTION; OLDER; MASS; STRENGTH; RESISTANCE; ADIPOSITY; SURVIVAL;
D O I
10.1371/journal.pone.0211629
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Reports using computed tomography (CT) to estimate thigh skeletal muscle cross-sectional area and mean muscle attenuation are often difficult to evaluate due to inconsistent methods of quantification and/or poorly described analysis methods. This CT tutorial provides step-by-step instructions in using free, NIH Image J software to quantify both muscle size and composition in the mid-thigh, which was validated against a robust commercially available software, SliceOmatic. CT scans of the mid-thigh were analyzed from 101 healthy individuals aged 65 and older. Mean cross-sectional area and mean attenuation values are presented across seven defined Hounsfield unit (HU) ranges along with the percent contribution of each region to the total mid-thigh area. Inter-software correlation coefficients ranged from R-2 = 0.92-0.99 for all specific area comparisons measured using the Image J method compared to SliceOmatic. We recommend reporting individual HU ranges for all areas measured. Although HU range 0-100 includes the majority of skeletal muscle area, HU range -29 to 150 appears to be the most inclusive for quantifying total thigh muscle. Reporting all HU ranges is necessary to determine the relative contribution of each, as they may be differentially affected by age, obesity, disease, and exercise. This standardized operating procedure will facilitate consistency among investigators reporting computed tomography characteristics of the thigh on single slice images.
引用
收藏
页数:11
相关论文
共 19 条
[1]   Measurement of skeletal muscle radiation attenuation and basis of its biological variation [J].
Aubrey, J. ;
Esfandiari, N. ;
Baracos, V. E. ;
Buteau, F. A. ;
Frenette, J. ;
Putman, C. T. ;
Mazurak, V. C. .
ACTA PHYSIOLOGICA, 2014, 210 (03) :489-497
[2]   Clinical outcomes related to muscle mass in humans with cancer and catabolic illnesses [J].
Baracos, Vickie ;
Kazemi-Bajestani, Seyyed Mohammad Reza .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2013, 45 (10) :2302-2308
[3]   Clustering of Strength, Physical Function, Muscle, and Adiposity Characteristics and Risk of Disability in Older Adults [J].
Cawthon, Peggy M. ;
Fox, Kathleen M. ;
Gandra, Shravanthi R. ;
Delmonico, Matthew J. ;
Chiou, Chiun-Fang ;
Anthony, Mary S. ;
Caserotti, Paolo ;
Kritchevsky, Stephen B. ;
Newman, Anne B. ;
Goodpaster, Bret H. ;
Satterfield, Suzanne ;
Cummings, Steven R. ;
Harris, Tamara B. .
JOURNAL OF THE AMERICAN GERIATRICS SOCIETY, 2011, 59 (05) :781-787
[4]   Efficacy of Whey Protein Supplementation on Resistance ExerciseInduced Changes in Lean Mass, Muscle Strength, and Physical Function in Mobility-Limited Older Adults [J].
Chale, Angela ;
Cloutier, Gregory J. ;
Hau, Cynthia ;
Phillips, Edward M. ;
Dallal, Gerard E. ;
Fielding, Roger A. .
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 2013, 68 (06) :682-690
[5]   Moderate Exercise Attenuates the Loss of Skeletal Muscle Mass That Occurs With Intentional Caloric Restriction-Induced Weight Loss in Older, Overweight to Obese Adults [J].
Chomentowski, Peter ;
Dube, John J. ;
Amati, Francesca ;
Stefanovic-Racic, Maja ;
Zhu, Shanjian ;
Toledo, Frederico G. S. ;
Goodpaster, Bret H. .
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 2009, 64 (05) :575-580
[6]   Effective exercise modality to reduce insulin resistance in women with type 2 diabetes [J].
Cuff, DJ ;
Meneilly, GS ;
Martin, A ;
Ignaszewski, A ;
Tildesley, HD ;
Frohlich, JJ .
DIABETES CARE, 2003, 26 (11) :2977-2982
[7]   Tutorial for using SliceOmatic to calculate thigh area and composition from computed tomography images from older adults [J].
Dennis, Richard A. ;
Long, Douglas E. ;
Landes, Reid D. ;
Padala, Kalpana P. ;
Padala, Prasad R. ;
Garner, Kimberly K. ;
Wise, James N. ;
Peterson, Charlotte A. ;
Sullivan, Dennis H. .
PLOS ONE, 2018, 13 (10)
[8]   Measuring Abdominal Circumference and Skeletal Muscle From a Single Cross-Sectional Computed Tomography Image: A Step-by-Step Guide for Clinicians Using National Institutes of Health ImageJ [J].
Gomez-Perez, Sandra L. ;
Haus, Jacob M. ;
Sheean, Patricia ;
Patel, Bimal ;
Mar, Winnie ;
Chaudhry, Vivek ;
McKeever, Liam ;
Braunschweig, Carol .
JOURNAL OF PARENTERAL AND ENTERAL NUTRITION, 2016, 40 (03) :308-318
[9]   Skeletal muscle attenuation determined by computed tomography is associated with skeletal muscle lipid content [J].
Goodpaster, BH ;
Kelley, DE ;
Thaete, FL ;
He, J ;
Ross, R .
JOURNAL OF APPLIED PHYSIOLOGY, 2000, 89 (01) :104-110
[10]  
Goodpaster BH, 2000, ANN NY ACAD SCI, V904, P18