Genetic diversity studies in pea (Pisum sativum L.) using simple sequence repeat markers

被引：18
|
作者：
Kumari, P. [1 ]
Basal, N. [1 ]
Singh, A. K. [1 ]
Rai, V. P. [1 ]
Srivastava, C. P. [1 ]
Singh, P. K. [1 ]
机构：
[1] Banaras Hindu Univ, Inst Agr Sci, Dept Genet & Plant Breeding, Varanasi 221005, Uttar Pradesh, India
来源：
GENETICS AND MOLECULAR RESEARCH | 2013年 / 12卷 / 03期
关键词：
Pea; Diversity; Molecular markers; Dendrogram; Polymorphism information content; RAPD TECHNIQUE; MICROSATELLITE; IDENTIFICATION; POLYMORPHISM; VARIETIES; GENOTYPES; PEDIGREE; PRIMERS;
D O I：
10.4238/2013.March.13.12
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The genetic diversity among 28 pea (Pisum sativum L.) genotypes was analyzed using 32 simple sequence repeat markers. A total of 44 polymorphic bands, with an average of 2.1 bands per primer, were obtained. The polymorphism information content ranged from 0.657 to 0.309 with an average of 0.493. The variation in genetic diversity among these cultivars ranged from 0.11 to 0.73. Cluster analysis based on Jaccard's similarity coefficient using the unweighted pair-group method with arithmetic mean (UPGMA) revealed 2 distinct clusters, I and II, comprising 6 and 22 genotypes, respectively. Cluster II was further differentiated into 2 subclusters, IIA and IIB, with 12 and 10 genotypes, respectively. Principal component (PC) analysis revealed results similar to those of UPGMA. The first, second, and third PCs contributed 21.6, 16.1, and 14.0% of the variation, respectively; cumulative variation of the first 3 PCs was 51.7%.
引用
收藏
页码：3540 / 3550
页数：11
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