Ferulic acid 5-hydroxylase 1 is essential for expression of anthocyanin biosynthesis-associated genes and anthocyanin accumulation under photooxidative stress in Arabidopsis

被引：30

作者：

Maruta, Takanori ^{[1
,2
]}

Noshi, Masahiro ^{[1
]}

Nakamura, Maki ^{[1
]}

Matsuda, Shun ^{[1
]}

Tamoi, Masahiro ^{[1
]}

Ishikawa, Takahiro ^{[2
]}

Shigeoka, Shigeru ^{[1
]}

机构：

[1] Kinki Univ, Fac Agr, Dept Adv Biosci, Nara 6318505, Japan

[2] Shimane Univ, Fac Life & Environm Sci, Dept Life Sci & Biotechnol, Matsue, Shimane 6908504, Japan

来源：

PLANT SCIENCE | 2014年 / 219卷

基金：

日本学术振兴会;

关键词：

Reactive oxygen species; Photooxidative stress; Ferulic acid 5-hydroxylase 1; Anthocyanin; Phenylpropanoid pathway; ASCORBATE PEROXIDASE; OXIDATIVE STRESS; TRANSCRIPTION FACTOR; CHLOROPLASTS; REGULATOR; RESPONSES; NUCLEUS; H2O2;

D O I：

10.1016/j.plantsci.2014.01.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anthocyanins are important for preventing photoinhibition and photodamage. By comprehensive reverse genetic analysis of chloroplast-produced H2O2-responsive genes, we isolated here an anthocyanin-deficient mutant under photooxidative stress, which lacked ferulate 5-hydroxylase 1 (FAH1) involved in the phenylpropanoid pathway. Interestingly, the expression of anthocyanin biosynthesis-associated genes was also inhibited in this mutant. These findings suggest that FAH1 is essential for expression of anthocyanin biosynthesis-associated genes and anthocyanin accumulation under photooxidative stress in Arabidopsis. Furthermore, we found that estrogen-inducible silencing of thylakoid membrane-bound ascorbate peroxidase, which is a major H2O2-scavenging enzyme in chloroplasts, enhances the expression of FAH1 and anthocyanin biosynthesis-associated genes and accumulation of anthocyanin without any application of stress. Thus, it is likely that chloroplastic H2O2 activates FAH1 expression to induce anthocyanin accumulation for protecting cells from photooxidative stress. (c) 2014 Elsevier Ireland Ltd. All rights reserved.

引用

页码：61 / 68

页数：8

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