Trends in metal-binding and metalloprotein analysis

被引：124
作者：
Garcia, JS [1 ]
de Magalhaes, CS [1 ]
Arruda, MAZ [1 ]
机构：
[1] Univ Estadual Campinas, Inst Chem, Dept Analyt Chem, BR-13084971 Campinas, SP, Brazil
来源：
TALANTA | 2006年 / 69卷 / 01期
基金：
巴西圣保罗研究基金会;
关键词：
metal-binding protein; metalloprotein; separation techniques; determination techniques;
D O I：
10.1016/j.talanta.2005.08.041
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
This review describes recent tendencies for metal-binding and metalloprotein analysis, emphasizing metal quantification in proteins through X-ray, atomic absorption, mass spectrometric techniques, and others. Hyphenated techniques such as capillary electrophoresis-synchrotron radiation X-ray fluorescence (CE-SRXRF), laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS), matrix-assisted laser desorption/ionisation-time-of-flight mass spectrometry (MALDI-TOF-MS), etc. are also presented. As protein separation techniques electrophoresis (mainly sodium dodecyl sulphate-polyacrylamide gel electrophoresis, SDS-PAGE), capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) are indicated, due to their inherent sensitivity, resolution and/or easy implementation. Latest challenges in metallomics are also commented. (c) 2005 Elsevier B.V. All rights reserved.
引用
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页码：1 / 15
页数：15
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