Deterministic separation of cancer cells from blood at 10 mL/min

被引：218

作者：

Loutherback, Kevin ^{[1
,2
]}

D'Silva, Joseph ^{[1
,2
]}

Liu, Liyu ^{[1
,3
]}

Wu, Amy ^{[1
,2
]}

Austin, Robert H. ^{[1
,3
]}

Sturm, James C. ^{[1
,2
]}

机构：

[1] Princeton Univ, Princeton Inst Sci & Technol Mat PRISM, Princeton, NJ 08544 USA

[2] Princeton Univ, Dept Elect Engn, Princeton, NJ 08544 USA

[3] Princeton Univ, Dept Phys, Princeton, NJ 08544 USA

来源：

AIP ADVANCES | 2012年 / 2卷 / 04期

关键词：

CIRCULATING TUMOR-CELLS; BREAST-CANCER; METASTASIS; SIZE; SOIL;

D O I：

10.1063/1.4758131

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Circulating tumor cells (CTCs) and circulating clusters of cancer and stromal cells have been identified in the blood of patients with malignant cancer and can be used as a diagnostic for disease severity, assess the efficacy of different treatment strategies and possibly determine the eventual location of metastatic invasions for possible treatment. There is thus a critical need to isolate, propagate and characterize viable CTCs and clusters of cancer cells with their associated stroma cells. Here, we present a microfluidic device for mL/min flow rate, continuous-flow capture of viable CTCs from blood using deterministic lateral displacement (DLD) arrays. We show here that a DLD array device can isolate CTCs from blood with capture efficiency greater than 85% CTCs at volumetric flow rates of up to 10 mL/min with no effect on cell viability. Copyright 2012 Author(s). This article is distributed under a Creative Commons Attribution 3.0 Unported License. [http://dx.doi.org/10.1063/1.4758131]

引用

页数：7

共 30 条

[1] Intravascular origin of metastasis from the proliferation of endothelium-attached tumor cells: a new model for metastasis [J].