Interaction between hemoglobin A and merocyanine 540: A spectroscopic investigation supported by docking

被引：13
作者：
Banerjee, Mousumi [1 ]
Chakrabarti, Abhijit [2 ]
Basu, Samita [1 ]
机构：
[1] Saha Inst Nucl Phys, Div Chem Sci, Kolkata 700064, India
[2] Saha Inst Nucl Phys, Struct Genom Div, Kolkata 700064, India
来源：
DYES AND PIGMENTS | 2013年 / 97卷 / 03期
关键词：
Hemoglobin A; Merocyanine; 540; Circular dichroism; Synchronous fluorescence; Forster resonance energy transfer; Binding constant; HUMAN SERUM-ALBUMIN; AUTOMATED DOCKING; CIRCULAR-DICHROISM; BINDING; FLUORESCENCE; PROTEINS; CELLS;
D O I：
10.1016/j.dyepig.2013.01.005
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Merocyanine 540 (MC 540) is a clinically important dye and a potent sensitizer of lipid peroxidation in natural cell membrane like erythrocyte ghost. We have studied the binding interaction between this antileukemic drug and Hemoglobin A (HbA) using UV-visible absorption, steady-state, time-resolved fluorescence and circular dichroism spectroscopy. The changes in absorption spectra of HbA in presence of MC 540 suggest a ground state complex formation between them. Thermodynamic analyses of quenching of HbA with MC 540 at different temperatures imply that the interaction is spontaneous and H-bonding as well as van der Waals interactions play the key role in this particular interaction. The binding constant and stoichiometry of the complex are 2.89 x 10(4) M-1 and 1.0 respectively at 298 K. Circular dichroism and synchronous fluorescence spectra suggest a structural change in HbA in presence of MC 540. Theoretical docking study helps to find out the plausible binding site of MC 540 inside HbA. (c) 2013 Elsevier Ltd. All rights reserved.
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收藏
页码：446 / 454
页数：9
相关论文
共 54 条
[1]   Preferential inactivation of paediatric solid tumour cells by sequential exposure to Merocyanine 540-mediated photodynamic therapy and Edelfosine: implications for the ex vivo purging of autologous haematopoietic stem cell grafts [J].
Anderson, GS ;
Tsujino, I ;
Miyagi, K ;
Sampson, R ;
Sieber, F .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2003, 69 (02) :87-95
[2]   THE PHOTOPHYSICS OF MEROCYANINE 540 - A COMPARATIVE-STUDY IN ETHANOL AND IN LIPOSOMES [J].
ARAMENDIA, PF ;
KRIEG, M ;
NITSCH, C ;
BITTERSMANN, E ;
BRASLAVSKY, SE .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1988, 48 (02) :187-194
[3]  
Banerjee M, 2011, J INDIAN CHEM SOC, V88, P1
[4]   Oxidative Interaction between OxyHb and ATP: A Spectroscopic Study [J].
Banerjee, Mousumi ;
Chakrabarti, Abhijit ;
Basu, Samita .
JOURNAL OF PHYSICAL CHEMISTRY B, 2012, 116 (21) :6150-6157
[5]   Interaction of Merocyanine 540 with serum albumins: Photophysical and binding studies [J].
Banerjee, Mousumi ;
Pal, Uttam ;
Subudhhi, Arijita ;
Chakrabarti, Abhijit ;
Basu, Samita .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2012, 108 :23-33
[6]   Simultaneous Occurrence of Energy Transfer and Photoinduced Electron Transfer in Interactions of Hen Egg White Lysozyme with 4-Nitroquinoline-1-Oxide [J].
Banerjee, Mousumi ;
Maiti, Sourav ;
Kundu, Ipsita ;
Chakrabarti, Abhijit ;
Basu, Samita .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 2010, 86 (06) :1237-1246
[7]   BIOMONITORING OF AROMATIC-AMINES .3. HEMOGLOBIN BINDING OF BENZIDINE AND SOME BENZIDINE CONGENERS [J].
BIRNER, G ;
ALBRECHT, W ;
NEUMANN, HG .
ARCHIVES OF TOXICOLOGY, 1990, 64 (02) :97-102
[8]   Deferiprone (L1) induced conformation change of hemoglobin: A fluorescence and CD spectroscopic study [J].
Chakraborty, D ;
Bhattacharyya, M .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2000, 204 (1-2) :17-20
[9]   Photophysical evaluation of mTHPC-loaded HSA nanoparticles as novel PDT delivery systems [J].
Chen, Kuan ;
Wacker, Matthias ;
Hackbarth, Steffen ;
Ludwig, Carmen ;
Langer, Klaus ;
Roder, Beate .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2010, 101 (03) :340-347
[10]   DETERMINATION OF SECONDARY STRUCTURES OF PROTEINS BY CIRCULAR-DICHROISM AND OPTICAL ROTATORY DISPERSION [J].
CHEN, YH ;
YANG, JT ;
MARTINEZ, HM .
BIOCHEMISTRY, 1972, 11 (22) :4120-+
123456