Preliminary in vitro and in vivo assessment of modified collagen/hydroxyapatite composite

被引：9

作者：

Kozlowska, Justyna ^{[1
]}

Jundzill, Arkadiusz ^{[2
]}

Bajek, Anna ^{[2
]}

Bodnar, Magdalena ^{[3
]}

Marszalek, Andrzej ^{[3
]}

Witmanowski, Henryk ^{[4
]}

Sionkowska, Alina ^{[1
]}

机构：

[1] Nicolaus Copernicus Univ Torun, Fac Chem, Torun, Poland

[2] Nicolaus Copernicus Univ Torun, Ludwik Rydygier Coll Med Bydgoszcz, Fac Med, Chair Urol, Bydgoszcz, Poland

[3] Nicolaus Copernicus Univ Torun, Ludwik Rydygier Coll Med Bydgoszcz, Dept Clin Pathomorphol, Bydgoszcz, Poland

[4] Nicolaus Copernicus Univ Torun, Ludwik Rydygier Coll Med Bydgoszcz, Chair & Clin Plast Reconstruct & Aesthet Surg, Bydgoszcz, Poland

来源：

MATERIALS LETTERS | 2018年 / 221卷

关键词：

Collagen; Hydroxyapatite; Composite; Scaffold; Biocompatibility; COLLAGEN; BIOMATERIALS; SCAFFOLDS; POLYMERS; BLENDS;

D O I：

10.1016/j.matlet.2018.03.122

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

In this work a porous collagen/hydroxyapatite matrices were fabricated by the freeze-drying method and then modified using a dehydrothermal treatment. The aim of present study was to investigate the biological properties of prepared scaffolds. Both in vitro as well as in vivo methods were used for these studies. The in vitro study were performed using mouse fibroblasts cells line NIH 3T3 and in vivo by subdermal and peritoneal cavity implantation in rats for up 30 days. The results of in vitro cytotoxity tests indicated that the samples were well tolerated by the cells. Good biocompatibility is demonstrated by the absence of necrosis or abscess formation in the surrounding tissues. This study provides valuable insight, which may lead to new applications of collagen/hydroxyapatite matrices in the field of tissue engineering. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：74 / 76

页数：3

共 20 条

[1] Effects of photochemical crosslinking on the microstructure of collagen and a feasibility study on controlled protein release [J].

Chan, B. P. ;

Chan, O. C. M. ;

So, K. -F. .

ACTA BIOMATERIALIA, 2008, 4 (06) :1627-1636

[2] Collagen hydrolysate based collagen/hydroxyapatite composite materials [J].

Ficai, Anton ;

Albu, Madalina Georgiana ;

Birsan, Mihaela ;

Sonmez, Maria ;

Ficai, Denisa ;

Trandafir, Viorica ;

Andronescu, Ecaterina .

JOURNAL OF MOLECULAR STRUCTURE, 2013, 1037 :154-159

[3] Hydroxyapatite reinforced collagen scaffolds with improved architecture and mechanical properties [J].

Kane, Robert J. ;

Weiss-Bilka, Holly E. ;

Meagher, Matthew J. ;

Liu, Yongxing ;

Gargac, Joshua A. ;

Niebur, Glen L. ;

Wagner, Diane R. ;

Roeder, Ryan K. .

ACTA BIOMATERIALIA, 2015, 17 :16-25

[4] Biomedical applications of collagen [J].

Lee, CH ;

Singla, A ;

Lee, Y .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2001, 221 (1-2) :1-22

[5] Biomaterials for Tissue Engineering [J].

Lee, Esther J. ;

Kasper, F. Kurtis ;

Mikos, Antonios G. .

ANNALS OF BIOMEDICAL ENGINEERING, 2014, 42 (02) :323-337

[6] Carbodiimide crosslinked collagen from porcine dermal matrix for high-strength tissue engineering scaffold [J].

Li, Jianhua ;

Ren, Na ;

Qiu, Jichuan ;

Jiang, Huaidong ;

Zhao, Hongshi ;

Wang, Guancong ;

Boughton, Robert I. ;

Wang, Yingjun ;

Liu, Hong .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2013, 61 :69-74

[7] Tissue Engineering Strategies for the Regeneration of Orthopedic Interfaces [J].

Lu, Helen H. ;

Subramony, Siddarth D. ;

Boushell, Margaret K. ;

Zhang, Xinzhi .

ANNALS OF BIOMEDICAL ENGINEERING, 2010, 38 (06) :2142-2154

[8] Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration [J].

Meghezi, Sebastien ;

Seifu, Dawit G. ;

Bono, Nina ;

Unsworth, Larry ;

Mequanint, Kibret ;

Mantovani, Diego .

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2015, (100)

[9] Biodegradable polymers as biomaterials [J].

Nair, Lakshmi S. ;

Laurencin, Cato T. .

PROGRESS IN POLYMER SCIENCE, 2007, 32 (8-9) :762-798

[10]

Silver F. H., BIOMATERIALS SCI BIO

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