Quantification of circulating cell-free plasma DNA and endothelial gene RNA in patients with burns and relation to acute thermal injury

被引：22

作者：

Fox, A. ^{[2
,3
]}

Gal, S. ^{[4
]}

Fisher, N. ^{[2
]}

Smythe, J. ^{[2
]}

Wainscoat, J. ^{[4
]}

Tyler, M. P. H. ^{[3
]}

Watt, S. M. ^{[2
]}

Harris, A. L. ^{[1
]}

机构：

[1] Univ Oxford, Weatherall Inst Mol Med, John Radcliffe Hosp, CRUK Clin Canc Ctr, Oxford OX3 9DS, England

[2] NHS Blood & Transplant Serv, Stem Cells & Immunotherapies Lab, Oxford, England

[3] Stoke Mandeville Hosp, Dept Burns Plast & Reconstruct Surg, Aylesbury HP21 8AL, Bucks, England

[4] Univ Oxford, John Radcliffe Hosp, Nuffield Dept, Clin Sci Lab, Oxford OX3 9DU, England

来源：

BURNS | 2008年 / 34卷 / 06期

关键词：

thermal injury; circulating nucleic acids; injury severity; endothelial progenitor cells;

D O I：

10.1016/j.burns.2007.10.003

中图分类号：

R4 [临床医学];

学科分类号：

1002 ; 100602 ;

摘要：

Background: Major burn represents a multi-system insult to the human body. Despite improvements in mortality and morbidity, reliable predictors of outcome are lacking. Raised levels of cell-free nucleic acids have been detected in various pathological processes including bums. We quantified circulating nucleic acids as potential objective measures of burn severity with predictive and prognostic value. Methods: Expression of endothelial specific cell-free mRNA and cell-free DNA were measured in plasma of 19 burn patients at days 1-3 and week 10 following acute thermal injury and in 19 healthy controls by real-time quantitative PCR. Results: Expression of endothelial specific mRNA was higher in burn patients compared to controls (p < 0.001), DNA levels were significantly higher in the burn population in the first 48 h following injury. Plasma RNA and DNA levels related to %TBSA burn in the first 24 h and to the levels of circulating endothelial progenitor cells. Conclusions: We show that plasma levels of endothelial specific mRNA and DNA are elevated acutely following burns, and relate to severity in terms of %TBSA burnt. (C) 2007 Elsevier Ltd and ISBI. All rights reserved.

引用

页码：809 / 816

页数：8