Functional genomics of probiotic Lactobacilli

被引：61

作者：

Klaenhammer, Todd R. ^{[1
]}

Altermann, Eric ^{[1
]}

Pfeiler, Erika ^{[1
]}

Buck, Brock Logan ^{[1
]}

Goh, Yong-Jun ^{[1
]}

O'Flaherty, Sarah ^{[1
]}

Barrangou, Rodolphe ^{[1
]}

Duong, Tri ^{[1
]}

机构：

[1] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA

来源：

JOURNAL OF CLINICAL GASTROENTEROLOGY | 2008年 / 42卷 / 08期

关键词：

Lactobacillus acidophilus; Lactobacillus johnsonii; Lactobacillus plantarum; Lactobacillus gasseri; genomics; probiotic cultures; lactic acid bacteria;

D O I：

10.1097/MCG.0b013e31817da140

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Lactic acid bacteria (LAB) have been used in fermentation processes for millennia. Recent applications such as the use of living cultures as probiotics have significantly increased industrial interest. Related bacterial strains can differ significantly in their genotype and phenotype, and features from one bacterial strain or species cannot necessarily be applied to a related one. These strain or family-specific differences often represent unique and applicable traits. Since 2002, the complete genomes of 13 probiotic LABs have been published. The presentation will discuss these genomes and highlight probiotic traits that are predicted, or functionally linked to genetic content. We have conducted a comparative genomic analysis of 4 completely sequenced Lactobacillus strains versus 25 lactic acid bacterial genomes present in the public database at the time of analysis. Using Differential Blast Analysis, each genome is compared with 3 other Lactobacillus and 25 other LAB genomes. Differential Blast Analysis highlighted strain-specific genes that were not represented in any other LAB used in this analysis and also identified group-specific genes shared within lactobacilli. Lactobacillus-specific genes include mucus-binding proteins involved in cell-adbesion and several transport systems for carbohydrates and amino acids. Comparative genomic analysis has identified gene targets in Lactobacillus acidophilus for functional analysis, including adhesion to mucin and intestinal epithelial cells, acid tolerance, bile tolerance, and quorum sensing. Whole genome transcriptional profiling of L. acidophilus, and isogenic mutants thereof, has revealed the impact of varying conditions (pH, bile, carbohydrates) and food matrices on the expression of genes important to probiotic-linked mechanisms.

引用

页码：S160 / S162

页数：3

共 15 条

[1] Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM [J].

Altermann, E ;

Russell, WM ;

Azcarate-Peril, MA ;

Barrangou, R ;

Buck, BL ;

McAuliffe, O ;

Souther, N ;

Dobson, A ;

Duong, T ;

Callanan, M ;

Lick, S ;

Hamrick, A ;

Cano, R ;

Klaenhammer, TR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (11) :3906-3912

[2] Global analysis of carbohydrate utilization by Lactobacillus acidophilus using cDNA microarrays [J].

Barrangou, R ;

Azcarate-Peril, MA ;

Duong, T ;

Conners, SB ;

Kelly, RM ;

Klaenhammer, TR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (10) :3816-3821

[3] Functional and comparative genomic analyses of an operon involved in fructooligosaccharide utilization by Lactobacillus acidophilus [J].

Barrangou, R ;

Altermann, E ;

Hutkins, R ;

Cano, R ;

Klaenhammer, TR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (15) :8957-8962

[4] Complete sequence and comparative genome analysis of the dairy bacterium Streptococcus thermophilus [J].

Bolotin, A ;

Quinquis, B ;

Renault, P ;

Sorokin, A ;

Ehrlich, SD ;

Kulakauskas, S ;

Lapidus, A ;

Goltsman, E ;

Mazur, M ;

Pusch, GD ;

Fonstein, M ;

Overbeek, R ;

Kyprides, N ;

Purnelle, B ;

Prozzi, D ;

Ngui, K ;

Masuy, D ;

Hancy, F ;

Burteau, S ;

Boutry, M ;

Delcour, J ;

Goffeau, A ;

Hols, P .

NATURE BIOTECHNOLOGY, 2004, 22 (12) :1554-1558

[5] The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp lactis IL1403 [J].

Bolotin, A ;

Wincker, P ;

Mauger, S ;

Jaillon, O ;

Malarme, K ;

Weissenbach, J ;

Ehrlich, SD ;

Sorokin, A .

GENOME RESEARCH, 2001, 11 (05) :731-753

[6] The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K [J].

Chaillou, S ;

Champomier-Vergès, MC ;

Cornet, M ;

Crutz-Le Coq, AM ;

Dudez, AM ;

Martin, V ;

Beaufils, S ;

Darbon-Rongère, E ;

Bossy, R ;

Loux, V ;

Zagorec, M .

NATURE BIOTECHNOLOGY, 2005, 23 (12) :1527-1533

[7] Multireplicon genome architecture of Lactobacillus salivarius [J].

Claesson, MJ ;

Li, Y ;

Leahy, S ;

Canchaya, C ;

van Pijkeren, JP ;

Cerdeño-Tárraga, AM ;

Parkhill, J ;

Flynn, S ;

O'Sullivan, GC ;

Collins, JK ;

Higgins, D ;

Shanahan, F ;

Fitzgerald, GF ;

van Sinderen, D ;

O'Toole, PW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (17) :6718-6723

[8] The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution [J].

de Guchte, M. van ;

Penaud, S. ;

Grimaldi, C. ;

Barbe, V. ;

Bryson, K. ;

Nicolas, P. ;

Robert, C. ;

Oztas, S. ;

Mangenot, S. ;

Couloux, A. ;

Loux, V. ;

Dervyn, R. ;

Bossy, R. ;

Bolotin, A. ;

Batto, J. -M. ;

Walunas, T. ;

Gibrat, J. -F. ;

Bessieres, P. ;

Weissenbach, J. ;

Ehrlich, S. D. ;

Maguin, E. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (24) :9274-9279

[9]

Klaenhammer T.R., 2000, ENCY FOOD MICROBIOLO, V2, P1151

[10] Genomic features of lactic acid bacteria effecting bioprocessing and health [J].

Klaenhammer, TR ;

Barrangou, R ;

Buck, BL ;

Azcarate-Peril, MA ;

Altermann, E .

FEMS MICROBIOLOGY REVIEWS, 2005, 29 (03) :393-409

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