Gene probe-based detection of type E botulinum neurotoxin binding protein using polymerase chain reaction

被引：2

作者：

Singh, BR ^{[1
]}

BarcombCaddle, LA ^{[1
]}

Fu, FN ^{[1
]}

Li, BL ^{[1
]}

机构：

[1] UNIV MASSACHUSETTS,DEPT BIOL,N DARTMOUTH,MA 02747

来源：

TOXICON | 1996年 / 34卷 / 07期

关键词：

COMPLETE NUCLEOTIDE-SEQUENCE; CLOSTRIDIUM-BOTULINUM; NONTOXIC COMPONENT; PROGENITOR TOXIN;

D O I：

10.1016/0041-0101(95)00166-2

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Using primers based on the nucleotide sequence of a neurotoxin binding protein from Type E Clostridium botulinum cultures, an amplified DNA product was obtained through polymerase chain reaction. The 400 base pair amplified DNA fragment was detectable with as low as 0.1 pg template DNA from Type E C. botulinum, and its fidelity was confirmed by Southern blotting using a DNA probe designed to detect the expected amplified DNA fragment. On the other hand, no DNA amplification was observed with as high as 10 ng template DNA from related Types A and B C. botulinum or from C. tetani, indicating the specificity of the probe. Copyright (C) 1996 Elsevier Science Ltd

引用

页码：737 / 742

页数：6

共 18 条

[1]

BINZ T, 1990, J BIOL CHEM, V265, P9153

[2] NUCLEOTIDE-SEQUENCE OF THE GENE CODING FOR CLOSTRIDIUM-BOTULINUM (CLOSTRIDIUM-ARGENTINENSE) TYPE-G NEUROTOXIN - GENEALOGICAL COMPARISON WITH OTHER CLOSTRIDIAL NEUROTOXINS [J].