Characterization of Herpesvirus saimiri-transformed T lymphocytes from common variable immunodeficiency patients

Common variable immunodeficiency (CVID) is a very frequent but heterogeneous syndrome of antibody formation. The primary defect remains unknown, but many reports describe peripheral blood T lymphocyte dysfunctions in a substantial proportion of CVID patients, which may impair T-B cell collaboration. In order to investigate whether such putative defects were intrinsic to T cells or, rather, secondary to quantitative differences in T cell subset distribution, or to other described disorders, we have used Herpesvirus saimiri (HVS) for the targeted transformation of CVID CD4(+) and CD8(+) T cells and subsequent functional evaluation by flow cytometry of their capacity to generate cell surface (CD154, CD69) or soluble (IL-2, TNF-alpha, IFN-gamma) help after CD3 engagement. Unexpectedly, the results showed that 40 different CVID blood samples exposed to HVS gave rise with a significantly increased frequency to transformed CD4(+) T cell lines, compared to 40 age-matched controls (27% versus 3%, Pless than or equal to0.00002) suggesting the existence of a CVID-specific signalling difference which affects CD4(+) cell transformation efficiency. The functional analysis of 10 CD4(+) and 15 CD8(+) pure transformed T cell lines from CVID patients did not reveal any statistically significant difference as compared to controls. However, half of the CD4(+) transformed cell lines showed CD154 (but not CD69) induction (mean value of 46.8%) under the lower limit of the normal controls (mean value of 82.4%, Pless than or equal to0.0001). Exactly the same five cell lines showed, in addition, a significantly low induction of IL-2 (Pless than or equal to0.04), but not of TNF-alpha or IFN-gamma. None of these differences were observed in the remaining CD4(+) cell lines or in any of the transformed CD8(+) cell lines. We conclude that certain CVID patients show selective and intrinsic impairments for the generation of cell surface and soluble help by CD4(+) T cells, which may be relevant for B lymphocyte function. The transformed T cell lines will be useful to establish the biochemical mechanisms responsible for the described impairments.