CKC: Isolation of Nucleic Acids from a Diversity of Plants Using CTAB and Silica Columns

被引：24

作者：

Henderson, David C. ^{[1
]}

Hammond, John ^{[1
]}

机构：

[1] USDA ARS, US Natl Arboretum Floral & Nursery Plants Res Uni, Beltsville, MD 20705 USA

来源：

MOLECULAR BIOTECHNOLOGY | 2013年 / 53卷 / 02期

基金：

美国食品与农业研究所;

关键词：

RNA; DNA; Nucleic acid; CTAB; Silica; Plant virus; Plant pathogen; RNA ISOLATION METHOD; EFFICIENT METHOD; DNA ISOLATION; GENOMIC DNA; VIRUS; EXTRACTION; DIAGNOSIS; SEQUENCE; AMPLIFICATION; MICROARRAYS;

D O I：

10.1007/s12033-012-9494-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To assay for viruses in plant samples, we required a method for nucleic acid isolation that is rapid, simple, and applicable to the widest possible variety of plants. A protocol for isolation of total nucleic acid (TNA) was developed by combining common CTAB methods with silica spin columns. We report data on TNA purity and RNA quality from over 30 plant genera representing 25 families. Measurements showed that RNA is of high quality, and one-step RT-PCR was successfully performed on all samples. The protocol can be completed in less than 2 h.

引用

页码：109 / 117

页数：9

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