Discovery of pinoresinol reductase genes in sphingomonads

被引：32

作者：

Fukuhara, Y. ^{[1
]}

Kamimura, N. ^{[1
]}

Nakajima, M. ^{[2
]}

Hishiyama, S. ^{[3
]}

Hara, H. ^{[4
]}

Kasai, D. ^{[1
]}

Tsuji, Y. ^{[2
]}

Narita-Yamada, S. ^{[5
]}

Nakamura, S. ^{[5
]}

Katano, Y. ^{[5
]}

Fujita, N. ^{[5
]}

Katayama, Y. ^{[6
]}

Fukuda, M. ^{[1
]}

Kajita, S. ^{[2
]}

Masai, E. ^{[1
]}

机构：

[1] Nagaoka Univ Technol, Dept Bioengn, Nagaoka, Niigata 94021, Japan

[2] Tokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Koganei, Tokyo, Japan

[3] Forestry & Forest Prod Res Inst, Tsukuba, Ibaraki 305, Japan

[4] Okayama Univ Sci, Dept Biomed Engn, Kita Ku, Okayama, Japan

[5] Natl Inst Technol & Evaluat, Biol Resource Ctr, Shibuya Ku, Tokyo, Japan

[6] Nihon Univ, Coll Bioresource Sci, Fujisawa, Kanagawa, Japan

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2013年 / 52卷 / 01期

关键词：

Pinoresinol; Reductase; Lignin; Sphingomonads; SP STRAIN E6; LIGNIN-RELATED BIPHENYL; BETA-ARYL ETHER; PAUCIMOBILIS SYK-6; BIOFUEL PRODUCTION; CATABOLIC OPERON; BIOSYNTHESIS; DEGRADATION; PATHWAY; ENZYME;

D O I：

10.1016/j.enzmictec.2012.10.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacterial genes for the degradation of major dilignols produced in lignifying xylem are expected to be useful tools for the structural modification of lignin in plants. For this purpose, we isolated pinZ involved in the conversion of pinoresinol from Sphingobium sp. strain SYK-6. pinZ showed 43-77% identity at amino acid level with bacterial NmrA-like proteins of unknown function, a subgroup of atypical short chain dehydrogenases/reductases, but revealed only 15-21% identity with plant pinoresinol/lariciresinol reductases. PinZ completely converted racemic pinoresinol to lariciresinol, showing a specific activity of 46 +/- 3 U/mg in the presence of NADPH at 30 degrees C. In contrast, the activity for lariciresinol was negligible. This substrate preference is similar to a pinoresinol reductase. AtPrR1, of Arabidopsis thaliana; however, the specific activity of PinZ toward (+/-)-pinoresinol was significantly higher than that of AtPrR1. The role of pinZ and a pinZ ortholog of Novosphingobium aromaticivorans DSM 12444 were also characterized. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：38 / 43

页数：6

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