Microdetermination of proteins by resonance light scattering spectroscopy with bromophenol blue

被引：95
作者：
Ma, CQ [1 ]
Li, KA [1 ]
Tong, SY [1 ]
机构：
[1] BEIJING UNIV, DEPT CHEM, BEIJING 100871, PEOPLES R CHINA
来源：
ANALYTICAL BIOCHEMISTRY | 1996年 / 239卷 / 01期
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1006/abio.1996.0294
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Reaction of bromophenol blue with proteins results in an enhanced resonance light scattering at 334 nm. Based on this, a new quantitative determination method for proteins in the aqueous solution is established. This assay is characterized by high sensitivity (0.34-18.7 mu g/ml), short reaction time (>2 min), and simplicity (a one-step assay). Due to protein-to-protein variability, this method gave results higher than that of the bromocresol green assay in detection of human serum albumin. (C) 1996 Academic Press, Inc.
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页码：86 / 91
页数：6
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