In Situ Detection of Liver Cancer Biomarkers with Atomic Force Microscopy at a Single-molecule Level

被引：1
作者：
Tang, Bochong [1 ]
Li, Min [1 ]
Zhang, Xiao [1 ,2 ]
Wang, Jiaojiao [1 ,3 ]
Xie, Peng [1 ]
Zhao, Yuliang [1 ]
Wei, Zhongqing [1 ]
机构：
[1] Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China
[2] Shandong Univ Sci & Technol, Coll Sci, Qingdao 266590, Peoples R China
[3] Univ Chinese Acad Sci, Coll Mat Sci & Optoelect Technol, Beijing 100049, Peoples R China
来源：
CHEMISTRY LETTERS | 2013年 / 42卷 / 10期
关键词：
GRAPHENE OXIDE; NANOARRAYS; MARKERS; CELLS;
D O I：
10.1246/cl.130440
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
alpha-Fetoprotein (AFP), a surveillance biomarker of liver cancer, was directly detected via immunoassay at a single-molecule level using in situ atomic force microscopy (AFM). AFM size measurements confirmed the successful capture of single APP molecules by monoclonal antibody molecules on a one-to-one basis.
引用
收藏
页码：1154 / 1156
页数：3
相关论文
共 22 条
  • [1] AFP, PIVKAII, GP3, SCCA-1 and follisatin as surveillance biomarkers for hepatocellular cancer in non-alcoholic and alcoholic fatty liver disease
    Beale, Gary
    Chattopadhyay, Dipankar
    Gray, Joe
    Stewart, Stephen
    Hudson, Mark
    Day, Christopher
    Trerotoli, Paolo
    Giannelli, Gianluigi
    Manas, Derek
    Reeves, Helen
    [J]. BMC CANCER, 2008, 8 (1)
  • [2] Hage DS, 1999, ANAL CHEM, V71, p294R
  • [3] ULTRALARGE ATOMICALLY FLAT TEMPLATE-STRIPPED AU SURFACES FOR SCANNING PROBE MICROSCOPY
    HEGNER, M
    WAGNER, P
    SEMENZA, G
    [J]. SURFACE SCIENCE, 1993, 291 (1-2) : 39 - 46
  • [4] Near-Infrared-Fluorescence-Enhanced Molecular Imaging of Live Cells on Gold Substrates
    Hong, Guosong
    Tabakman, Scott M.
    Welsher, Kevin
    Chen, Zhuo
    Robinson, Joshua T.
    Wang, Hailiang
    Zhang, Bo
    Dai, Hongjie
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 50 (20) : 4644 - 4648
  • [5] PREPARATION OF GRAPHITIC OXIDE
    HUMMERS, WS
    OFFEMAN, RE
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1958, 80 (06) : 1339 - 1339
  • [6] Kaiser J, 2010, SCIENCE, V327, P1072, DOI [10.1126/science.327.5969.1072, 10.1126/science.327.5969.1074]
  • [7] Protein nanoarrays generated by dip-pen nanolithography
    Lee, KB
    Park, SJ
    Mirkin, CA
    Smith, JC
    Mrksich, M
    [J]. SCIENCE, 2002, 295 (5560) : 1702 - 1705
  • [8] The use of nanoarrays for highly sensitive and selective detection of human immunodeficiency virus type 1 in plasma
    Lee, KB
    Kim, EY
    Mirkin, CA
    Wolinsky, SM
    [J]. NANO LETTERS, 2004, 4 (10) : 1869 - 1872
  • [9] In situ single-molecule detection of antibody-antigen binding by tapping-mode atomic force microscopy
    Li, LY
    Chen, SF
    Oh, SJ
    Jiang, SY
    [J]. ANALYTICAL CHEMISTRY, 2002, 74 (23) : 6017 - 6022
  • [10] The controlled evolution of a polymer single crystal
    Liu, XG
    Zhang, Y
    Goswami, DK
    Okasinski, JS
    Salaita, K
    Sun, P
    Bedzyk, MJ
    Mirkin, CA
    [J]. SCIENCE, 2005, 307 (5716) : 1763 - 1766
123