Genotoxic effects of produced waters in mosquito fish (Gambusia affinis)

被引：35

作者：

Caliani, Ilaria ^{[1
]}

Porcelloni, Serena ^{[1
]}

Mori, Gabriele ^{[1
]}

Frenzilli, Giada ^{[2
]}

Ferraro, Maria ^{[1
]}

Marsili, Letizia ^{[1
]}

Casini, Silvia ^{[1
]}

Fossi, Maria Cristina ^{[1
]}

机构：

[1] Univ Siena, Dept Environm Sci, I-53100 Siena, Italy

[2] Univ Pisa, Dept Human Morphol & Appl Biol, Pisa, Italy

来源：

ECOTOXICOLOGY | 2009年 / 18卷 / 01期

关键词：

Genotoxicity; Mosquito fish; PAHs; Micronucleus; Comet assay; DNA-DAMAGE; COMET ASSAY; IN-SITU; LIVER; L; ABERRATIONS; STRESS; SEA; OIL;

D O I：

10.1007/s10646-008-0259-0

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

The aim of this study was to assess the potential genotoxic effects of produced water (PW) from an Italian on-shore oil plant. Produced water is a complex mixture containing residual hydrocarbons, trace elements, naturally occurring radioactive material and potentially toxic treatment chemicals such as biocides, dispersants, detergents and scale inhibitors used in oil production. The test organism, mosquito fish (Gambusia affinis), was divided into male and female groups and exposed for 8 days in the laboratory to 50% concentrations of different produced waters: PW before treatment and after settling treatment. The fish were also exposed to lower concentrations (10%) of the same PW for 30 days. DNA damage was evaluated in erythrocytes by single cell gel electrophoresis (Comet assay) and micronucleus test, while an oxidative stress biomarker, was assessed. Polycyclic aromatic hydrocarbons (PAH) metabolites in bile were also evaluated. A higher sensitivity in biomarker responses was found in females in comparison to males. An increase in DNA strand breaks was observed in both genders after 30 days exposure and a statistically significant increase of micronucleated cells was found in females after 8 days exposure. A positive correlation between presence of micronucleated cells and PAH metabolites in bile was also observed.

引用

页码：75 / 80

页数：6

共 26 条

[1]

AEBI H, 1984, METHOD ENZYMOL, V105, P121

[2] Oxidative stress and genotoxic effects in gill and kidney of Anguilla anguilla L. exposed to chromium with or without pre-exposure to β-naphthoflavone [J].

Ahmad, Iqbal ;

Maria, V. L. ;

Oliveira, M. ;

Pacheco, M. ;

Santos, M. A. .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2006, 608 (01) :16-28

[3]

[Anonymous], 2002, BIOACCUMULATION MARI

[4] SYNCHRONOUS FLUORESCENCE SPECTROMETRY OF FISH BILE - A RAPID SCREENING METHOD FOR THE BIOMONITORING OF PAH EXPOSURE [J].

ARIESE, F ;

KOK, SJ ;

VERKAIK, M ;

GOOIJER, C ;

VELTHORST, NH ;

HOFSTRAAT, JW .

AQUATIC TOXICOLOGY, 1993, 26 (3-4) :273-286

[5] DNA DAMAGE BY BENZO [ALPHA] PYRENE IN THE LIVER OF MOSQUITO FISH GAMBUSIA-AFFINIS [J].

BATEL, R ;

BIHARI, N ;

KURELEC, B ;

ZAHN, RK .

SCIENCE OF THE TOTAL ENVIRONMENT, 1985, 41 (03) :275-283

[6] DNA damage and biliary PAH metabolites in flatfish from Southern California bays and harbors, and the Channel Islands [J].

Brown, JS ;

Steinert, SA .

ECOLOGICAL INDICATORS, 2004, 3 (04) :263-274

[7] Use of biomarkers to investigate toxicological effects of produced water treated with conventional and innovative methods [J].

Casini, S. ;

Marsili, L. ;

Fossi, M. C. ;

Mori, G. ;

Bucalossi, D. ;

Porcelloni, S. ;

Caliani, I. ;

Stefanini, G. ;

Ferraro, M. ;

Alberti di Catenaja, C. .

MARINE ENVIRONMENTAL RESEARCH, 2006, 62 :S347-S351

[8] PRODUCTION OF MICRONUCLEI FROM CHROMOSOME-ABERRATIONS IN IRRADIATED CULTURES OF HUMAN LYMPHOCYTES [J].

COUNTRYMAN, PI ;

HEDDLE, JA .

MUTATION RESEARCH, 1976, 41 (2-3) :321-331

[9] DNA damage in eelpout (Zoarces viviparus) from Goteborg harbour [J].

Frenzilli, G ;

Scarcelli, V ;

Del Barga, I ;

Nigro, M ;

Förlin, L ;

Bolognesi, C ;

Sturve, J .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2004, 552 (1-2) :187-195

[10]

Frenzilli G, 1999, NEOPLASMA, V46, P6

← 1 2 3 →