Damage responses to salt stress in salt-tolerant cell line of wheat

A salt-tolerant cell line of wheat ( Triticum aestivum L,.) that could grow well under 1.5% NaCl stress was obtained by gradual increase of NaCl concentration. Under NaCl stress, the water content in the salt-tolerant cell line decreased less than that in the salt sensitive cell line served as control, while the H2O2 content and O-2(-) production rate in the control cell line increased more obviously than those in the salt-tolerant cell line. Meanwhile, the membrane relative permeability, membrane lipid peroxidation and deesterification increased more significantly than those in the salt-tolerant cell line. These results indicated that the damage of wheat cell lines caused by NaCl stress had dose relationship with active oxygen-mediated membrane lipid peroxidation and deesterification, and the resistance to the active oxygen mediated damage in the salt-tolerant cell line was remarkably higher than that in the control cell line.