Surface active agents (SAAs) are a class of compounds, which is used in variety of products such as detergents, fabric softeners, soaps, paints, adhesives, inks and anti-fogs. After the use of these products containing surfactants are disposed in water reservoirs. The separation and determination of surfactants from complex matrices become challenging for analytical chemists. The fundamentals on separation, preconcentration and analysis of surfactants employing different analytical instrumental techniques for qualitative and quantitative determination of surfactants in environmental samples are discussed. In addition, this compiled work enhanced our knowledge in learning about pathway mechanisms and the degree of their environmental loads. We also discussed the different aspects of method validation in the framework of quality control (QC) and quality assurance (QA). This review provides information on levels of SAAs in various environmental samples including soil, sediments, sewage wastewater, river wastewater and aerosols worldwide. (c) 2019 Elsevier B.V. All rights reserved.