Characterization of the aap1 gene of Agaricus bisporus, a homolog of the yeast YAP1

被引：7

作者：

Navarro, Pilar ^{[1
,2
]}

Billette, Christophe ^{[1
]}

Ferrer, Nathalie ^{[1
]}

Savoie, Jean-Michel ^{[1
]}

机构：

[1] INRA, UR1264 MycSA, F-33882 Villenave Domon, France

[2] Univ Veracruzana, Fac Ciencias Biol & Agropecuarias, Cordoba, Veracruz, Mexico

来源：

COMPTES RENDUS BIOLOGIES | 2014年 / 337卷 / 01期

关键词：

bZIP; AP-1-like transcription factor; Yap transcription factor; Temperature adaptation; Mushroom; SINGLE-NUCLEOTIDE POLYMORPHISMS; MULTIPLE SEQUENCE ALIGNMENT; HIGH-TEMPERATURE; TRANSCRIPTION; HEAT; THERMOTOLERANCE; IDENTIFICATION; POPULATION; MARKERS; VERSION;

D O I：

10.1016/j.crvi.2013.10.010

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The structure, homologies, polymorphism and expression profiles of a new gene, aap1, have been studied for precisely characterizing it and defining its putative involvement in thermo-tolerance of both vegetative mycelium growth and sporophore differentiation. Sequence polymorphism was analyzed in 3 homokaryons of A. bisporus and 24 strains having different abilities for mycelial growth at temperatures above 30 degrees C and for producing mature fruiting bodies at 25 degrees C. The level of gene expression was measured by real-time PCR both in vegetative mycelium after transfer from 25 to 32 degrees C and in primordia and fruiting bodies produced during cultures at 17 or 25 degrees C. The results indicated that aap1 gene belong to a new subfamily of the yeast YAP1 homologs. It is not a dominant contributor to the thermo-tolerance of A. bisporus, but the protein it encodes may be involved as an overall stress resistance transcription factor. The way Aap1 senses redox level differs from that of AP-1-like transcription factor Yap1. (C) 2013 Academie des sciences. Published by Elsevier Masson SAS. All rights reserved.

引用

页码：29 / 43

页数：15