A very low potential electrochemical detection of L-cysteine based on a glassy carbon electrode modified with multi-walled carbon nanotubes/gold nanorods

被引：78
作者：
dos Santos Silva, Francisco de Assis [1 ,4 ]
Angelo da Silva, Monique Gabriella [1 ]
Lima, Phabyanno Rodrigues [1 ,2 ,4 ]
Meneghetti, Mario Roberto [1 ]
Kubota, Lauro Tatsuo [3 ,4 ]
Fonseca Goulart, Marilia Oliveira [1 ,4 ]
机构：
[1] Univ Fed Alagoas, Inst Quim & Biotecnol, BR-57072970 Maceio, AL, Brazil
[2] IFAL, Inst Fed Educ Ciencia & Tecnol Alagoas, BR-57072970 Maceio, AL, Brazil
[3] Univ Estadual Campinas, Inst Quim, BR-13084971 Campinas, SP, Brazil
[4] Univ Estadual Campinas, Inst Nacl Ciencia & Tecnol Bioanalit, BR-13084971 Campinas, SP, Brazil
来源：
BIOSENSORS & BIOELECTRONICS | 2013年 / 50卷
关键词：
Gold nanorods; MWCNT; L-cysteine; Electrocatalysis; Chemically modified electrodes; GOLD NANOPARTICLES; AMINO-ACIDS; ELECTROCATALYTIC OXIDATION; OXIDE NANOPARTICLES; AMPEROMETRIC SENSOR; ASCORBIC-ACID; HIGH-DENSITY; SURFACE; FILM; GLUTATHIONE;
D O I：
10.1016/j.bios.2013.06.036
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A nanohybrid platform built with multi-walled carbon nanotubes and gold nanorods, prepared via a cationic surfactant-containing seed-mediated sequential growth process, in aqueous solution, on a glassy carbon substrate has been successfully developed to be used in the electrocatalytic oxidation of L-cysteine (Cys). The nanohybrid was characterized by transmission electron microscopy, Raman spectroscopy and electrochemical measurements. Cyclic voltammetry results had shown that the modified electrode allows the oxidation of Cys at a very low anodic potential (0.00 V vs. Ag/AgCl). The kinetic constant k(cat) for the catalytic oxidation of Cys was evaluated by chronoamperometry and provided a value of 5.6 x 10(4) L mol(-1) s(-1). The sensor presents a linear response range from 5.0 up to 200.0 mu mol L-1, detection limit of 8.25 nmol L-1 and a sensitivity of 120 nA L mu mol(-1). (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：202 / 209
页数：8
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