Risk assessment of deoxynivalenol in high-risk area of China by human biomonitoring using an improved high throughput UPLC-MS/MS method

被引：40

作者：

Deng, Chunli ^{[1
]}

Li, Chenglong ^{[1
]}

Zhou, Shuang ^{[1
]}

Wang, Xiaodan ^{[1
]}

Xu, Haibin ^{[1
]}

Wang, Dan ^{[1
]}

Gong, Yun Yun ^{[1
,2
]}

Routledge, Michael N. ^{[3
]}

Zhao, Yunfeng ^{[1
]}

Wu, Yongning ^{[1
]}

机构：

[1] Minist Hlth, Key Lab Food Safety Risk Assessment, China Natl Ctr Food Safety Risk Assessment, Beijing 100021, Peoples R China

[2] Univ Leeds, Sch Food Sci & Nutr, Leeds LS2 9JT, W Yorkshire, England

[3] Univ Leeds, Sch Med, Leeds LS2 9JT, W Yorkshire, England

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

基金：

中国国家自然科学基金;

关键词：

MYCOTOXIN EXPOSURE; URINARY DEOXYNIVALENOL; PREGNANT-WOMEN; DIETARY EXPOSURE; BIOMARKER; ADULTS; VALIDATION; METABOLITE; EXCRETION; CHILDREN;

D O I：

10.1038/s41598-018-22206-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A risk assessment of deoxynivalenol (DON) was recently conducted for the residents in Henan province, China, where wheat as the staple food are highly consumed. A high-throughput sensitive UPLC-MS/MS method following 96-well mu Elution solid-phase extraction (SPE) were developed and validated for the determination of DON biomarkers in human urine. Isotope labelled internal standard, C-13-DON, was used for accurate quantification. Urinary samples collected from 151 healthy Chinese aged 2-78 years were processed with and without enzyme hydrolysis to determine total and free biomarkers, respectively. DON, and de-epoxy-deoxynivalenol (DOM-1) to a lesser extent, can be frequently detected in these samples both with and without enzyme hydrolysis. Free DOM-1 was detected at low level in human urine for the first time. Total DON was detected in all samples with a mean concentration at 47.6 ng mL(-1). The mean and median probable daily intakes (PDI) for the whole participants, estimated to be 1.61 mu g/kg bw and 1.10 mu g/kg bw, both exceeded the PMTDI (1 mu g/kg bw/day), indicating a potential risk for the residents in this area, especially for children and adolescents.

引用

页数：9

共 53 条

[41] Dietary wheat reduction decreases the level of urinary deoxynivalenol in UK adults [J].