Reliability of Small Biopsy Samples Compared With Resected Specimens for the Determination of Programmed Death-Ligand 1 Expression in Non-Small-Cell Lung Cancer

被引：118

作者：

Kitazono, Satoru ^{[1
,2
]}

Fujiwara, Yutaka ^{[1
,3
]}

Tsuta, Koji ^{[4
]}

Utsumi, Hirofumi ^{[1
]}

Kanda, Shintaro ^{[1
]}

Horinouchi, Hidehito ^{[1
]}

Nokihara, Hiroshi ^{[1
]}

Yamamoto, Noboru ^{[1
,3
]}

Sasda, Shinji ^{[5
]}

Watanabe, Shun-ichi ^{[6
]}

Asamura, Hisao ^{[6
]}

Tamura, Tomohide ^{[1
]}

Ohe, Yuichiro ^{[1
]}

机构：

[1] Natl Canc Ctr, Dept Thorac Oncol, Tokyo 1040045, Japan

[2] Japanese Fdn Canc Res, Canc Inst Hosp, Dept Thorac Med Oncol, Tokyo, Japan

[3] Natl Canc Ctr, Exploratory Oncol Res & Clin Trial Ctr, Dept Expt Therapeut, Tokyo, Japan

[4] Natl Canc Ctr, Dept Pathol, Tokyo, Japan

[5] Natl Canc Ctr, Dept Endoscopy, Resp Endoscopy Div, Tokyo, Japan

[6] Natl Canc Ctr, Dept Thorac Surg, Tokyo, Japan

来源：

CLINICAL LUNG CANCER | 2015年 / 16卷 / 05期

关键词：

Concordance rate; Immunohistochemistry; Operative; Surgical procedures; 1ST-LINE TREATMENT; B7; FAMILY; PD-1; B7-H1; CHEMOTHERAPY; CLASSIFICATION; CRIZOTINIB; PATHWAY; ESCAPE; MEMBER;

D O I：

10.1016/j.cllc.2015.03.008

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Expression of programmed death-ligand 1 (PD-L1) in nonesmall-cell lung cancer (NSCLC) has mainly been examined using surgically resected specimens. We retrospectively evaluated the expression of PD-L1 using immunohistochemistry in 79 paired small biopsy and resected specimens of NSCLC. The concordance between the samples was good, with a concordance rate of 92.4% and k value of 0.8366. Background: Several studies have assessed the expression of programmed death-ligand 1 (PD-L1) in resected surgical specimens of non-small-cell lung cancer (NSCLC). However, the expression of PD-L1 in smaller biopsy samples of advanced NSCLC has not been reported. Patients and Methods: A total of 79 patients with NSCLC at our institution with available biopsy samples and resected specimens were retrospectively enrolled in the present study. PD-L1 expression was assessed by immunohistochemistry and scored using the hybrid scoring method. The concordance rates for the expression of PD-L1 between the 2 samples were analyzed. Results: The pathologic stage of the patients (51 men, 28 women; median age, 68 years) was stage I in 37, stage II in 18, and stage III in 24. The diagnostic procedures included transbronchial biopsy in 59, transbronchial needle aspiration biopsy in 14, and computed tomography (CT)-guided needle biopsy in 6. The positivity rate of PD-L1 in these samples was 38.0% (27 transbronchial biopsies, 6 transbronchial needle aspiration biopsies, 3 CT-guided needle biopsies) versus 35.4% in the resected specimens. The median hybrid score was 0 (range, 0-170), and the mean score was 28.7 +/- 43.4. Comparing the biopsy samples and resected specimens with a score of >= 1 as positive for PD-L1 staining, 6 tumors were discordant for PD-L1 expression and 73 were concordant, for a concordance rate of 92.4% and kappa value of 0.8366. Conclusion: PD-L1 status showed good concordance between the biopsy samples and resected specimens. These small samples, even those derived from transbronchial needle aspiration biopsies, appear adequate for the assessment of PD-L1 expression. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：385 / 390

页数：6

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