Characterization of microsatellites in the fungal plant pathogen, Sclerotinia sclerotiorum

被引:0
作者
Sirjusingh, C [1 ]
Kohn, LM [1 ]
机构
[1] Univ Toronto, Dept Bot, Mississauga, ON L5L 1C6, Canada
来源
MOLECULAR ECOLOGY NOTES | 2001年 / 1卷 / 04期
关键词
DNA fingerprints; multilocus haplotypes; Sclerotinia minor; Sclerotinia trifoliorum; white mold;
D O I
10.1046/j.1471-8278 .2001.00102.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Twenty-five primers produced unambiguous amplification products of 23 microsatellite-containing loci and two microsatellite-like polymorphic loci, with 2-10 alleles at each locus in the plant pathogenic fungus, Sclerotinia sclerotiorum. Haplotypes are polymorphic among individuals sharing the same DNA fingerprint and DNA sequence haplotype, facilitating epidemiological monitoring worldwide. Fourteen of these primers also successfully amplified the closely related S. trifoliorum and S. minor.
引用
收藏
页码:267 / 269
页数:3
相关论文
共 8 条
[1]   A method for designing primer sets for speciation studies in filamentous ascomycetes [J].
Carbone, I ;
Kohn, LM .
MYCOLOGIA, 1999, 91 (03) :553-556
[2]  
Carbone I, 1999, EVOLUTION, V53, P11, DOI [10.2307/2640916, 10.1111/j.1558-5646.1999.tb05329.x]
[3]   A microbial population-species interface: nested cladistic and coalescent inference with multilocus data [J].
Carbone, I ;
Kohn, LM .
MOLECULAR ECOLOGY, 2001, 10 (04) :947-964
[4]   CHARACTERIZATION OF (GT)N AND (CT)N MICROSATELLITES IN 2 INSECT SPECIES - APIS-MELLIFERA AND BOMBUS-TERRESTRIS [J].
ESTOUP, A ;
SOLIGNAC, M ;
HARRY, M ;
CORNUET, JM .
NUCLEIC ACIDS RESEARCH, 1993, 21 (06) :1427-1431
[5]   CLONAL DISPERSAL AND SPATIAL MIXING IN POPULATIONS OF THE PLANT-PATHOGENIC FUNGUS, SCLEROTINIA-SCLEROTIORUM [J].
KOHLI, Y ;
BRUNNER, LJ ;
YOELL, H ;
MILGROOM, MG ;
ANDERSON, JB ;
MORRALL, RAA ;
KOHN, LM .
MOLECULAR ECOLOGY, 1995, 4 (01) :69-77
[6]   MYCELIAL INCOMPATIBILITY AND MOLECULAR MARKERS IDENTIFY GENETIC-VARIABILITY IN FIELD POPULATIONS OF SCLEROTINIA-SCLEROTIORUM [J].
KOHN, LM ;
STASOVSKI, E ;
CARBONE, I ;
ROYER, J ;
ANDERSON, JB .
PHYTOPATHOLOGY, 1991, 81 (04) :480-485
[7]   ISOLATION OF SIMPLE-SEQUENCE LOCI FOR USE IN POLYMERASE CHAIN REACTION-BASED DNA FINGERPRINTING [J].
RASSMANN, K ;
SCHLOTTERER, C ;
TAUTZ, D .
ELECTROPHORESIS, 1991, 12 (2-3) :113-118
[8]  
Sambrook E, 1989, MOL CLONING LAB MANU