A Simple and High Resolution Ion-Pair HPLC Method for Separation and Simultaneous Determination of Nitrate and Thiocyanate in Different Water Samples

被引：3

作者：

Soleimani, Majid ^{[1
]}

Yamini, Yadollah ^{[2
]}

Rad, Farzane Mohazab ^{[1
]}

机构：

[1] Imam Khomeini Int Univ, Dept Chem, Qazvin, Iran

[2] Tarbiat Modares Univ, Dept Chem, Tehran, Iran

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2012年 / 50卷 / 09期

关键词：

INORGANIC ANIONS; CHROMATOGRAPHY; PHASE;

D O I：

10.1093/chromsci/bms078

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An ion-pair reversed-phase high-performance liquid chromatography method with isocratic elution and ultraviolet detection was developed and validated for the separation and simultaneous determination of nitrate and thiocyanate. The separation was performed on a C18 analytical column with mobile phase containing 0.08 mM hexadecyltrimethylammonium bromide as an ion-pair reagent, 40 mM of acetate buffer and 30 methanol at pH 3.2. The detection was monitored at 206 nm. The response was linear from 1 to 10 mM for nitrate, with a detection limit of 0.05 mM, and 1 to 10 mM for thiocyanate, with a detection limit of 0.31 mM. Calibration curves were found to be linear in these concentration ranges with correlation coefficient better than 0.99. © 2012 The Author. Published by Oxford University Press. All rights reserved.

引用

页码：826 / 830

页数：5

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