Characterization of the vasa gene in the Chinese mitten crab Eriocheir sinensis: A germ line molecular marker

被引：20

作者：

Wang, Qun ^{[1
]}

Fang, Di-An ^{[1
]}

Sun, Jiang-Lin ^{[1
]}

Wang, Yang ^{[1
]}

Wang, Juan ^{[1
]}

Liu, Li-Hua ^{[1
]}

机构：

[1] E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China

来源：

JOURNAL OF INSECT PHYSIOLOGY | 2012年 / 58卷 / 07期

关键词：

DEAD-box family; Eriocheir sinensis; Genetic expression; Germ line; vasa; BOX PROTEIN FAMILY; EXPRESSION PATTERN; RNA HELICASE; FC-VASA; CELL; SHRIMP; HOMOLOG; DIFFERENTIATION; HEPATOPANCREAS; METAZOANS;

D O I：

10.1016/j.jinsphys.2012.04.012

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

The vasa gene first identified in Drosophila encodes an ATP-dependent RNA helicase belonging to the DEAD-box family and is specifically expressed in germ line cells. In this study a full-length vasa gene homolog from the Chinese mitten crab (Eriocheir sinensis) was characterized, comprising of 2369 bp nucleotides with an open reading frame of 1866 bp encoding 621 amino acids. The putative protein was shown to contain eight conserved motifs belonging to the DEAD-box protein family and two zinc-finger domains (CCHC) and a Q-motif. Its sequence showed high similarity to vasa homologs of other species. The E. sinensis vasa (Es-vasa) mRNA expression was specific to the gonad and its temporal expression in the ovary and testes were significantly different between various developmental periods. By real-time qPCR analysis, Es-vasa mRNA transcripts were at the highest levels during periods of rapid development in the gonads (stage III-2 in ovaries and spermatocyte stage in testes) and gradually decreased as the gonads matured. In conclusion, we first identified the vasa gene from Chinese mitten crab. The specificity and pattern of Es-vasa expression in gonads indicates that it may be used as molecular marker for germ line in E. sinensis. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：960 / 965

页数：6

共 47 条

[11] Post-translational regulation by gustavus contributes to selective Vasa protein accumulation in multipotent cells during embryogenesis [J].

Gustafson, Eric A. ;

Yajima, Mamiko ;

Juliano, Celina E. ;

Wessel, Gary M. .

DEVELOPMENTAL BIOLOGY, 2011, 349 (02) :440-450

[12] DEAD-box helicases: Posttranslational regulation and function [J].

Gustafson, Eric A. ;

Wessel, Gary M. .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2010, 395 (01) :1-6

[13] A PROTEIN-COMPONENT OF DROSOPHILA POLAR GRANULES IS ENCODED BY VASA AND HAS EXTENSIVE SEQUENCE SIMILARITY TO ATP-DEPENDENT HELICASES [J].

HAY, B ;

JAN, LY ;

JAN, YN .

CELL, 1988, 55 (04) :577-587

[14] Mating behaviour and chemical communication in the invasive Chinese mitten crab Eriocheir sinensis [J].

Herborg, LM ;

Bentley, MG ;

Clare, AS ;

Last, KS .

JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY, 2006, 329 (01) :1-10

[15]

Ikenishi K, 1997, DEV GROWTH DIFFER, V39, P625

[16] Chasing relationships between nutrition and reproduction: A comparative transcriptome analysis of hepatopancreas and testis from Eriocheir sinensis [J].

Jiang, Hui ;

Yin, Yuxin ;

Zhang, Xiaowei ;

Hu, Songnian ;

Wang, Qun .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS, 2009, 4 (03) :227-234

[17] Functional Annotation and Analysis of Expressed Sequence Tags from the Hepatopancreas of Mitten Crab (Eriocheir sinensis) [J].

Jiang, Hui ;

Cai, Yi-Mei ;

Chen, Li-Qiao ;

Zhang, Xiao-Wei ;

Hu, Song-Nian ;

Wang, Qun .

MARINE BIOTECHNOLOGY, 2009, 11 (03) :317-326

[18]

Kalinina M V, 2008, Ontogenez, V39, P58

[19] PRIMARY STRUCTURE AND BINDING-ACTIVITY OF THE HNRNP U-PROTEIN - BINDING RNA THROUGH RGG BOX [J].

KILEDJIAN, M ;

DREYFUSS, G .

EMBO JOURNAL, 1992, 11 (07) :2655-2664

[20] Differential expression of vasa homologue gene in the germ cells during oogenesis and spermatogenesis in a teleost fish, tilapia, Oreochromis niloticus [J].

Kobayashi, T ;

Kajiura-Kobayashi, H ;

Nagahama, Y .

MECHANISMS OF DEVELOPMENT, 2000, 99 (1-2) :139-142

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