Study of Metabolic Profile of Rhizopus oryzae to Enhance Fumaric Acid Production Under Low pH Condition

被引：8

作者：

Liu, Ying ^{[1
,2
]}

Xu, Qing ^{[1
,3
]}

Lv, Chunwei ^{[1
]}

Yan, Caixia ^{[1
]}

Li, Shuang ^{[1
]}

Jiang, Ling ^{[1
]}

Huang, He ^{[1
]}

Ouyang, Pingkai ^{[1
]}

机构：

[1] Nanjing Tech Univ, State Key Lab Mat Oriented Chem Engn, Coll Biotechnol & Pharmaceut Engn, Nanjing 211816, Jiangsu, Peoples R China

[2] Sinopec Corp, Sci & Technol Dev Dept, Beijing 100728, Peoples R China

[3] Tianjin Univ, Sch Chem Engn & Technol, Dept Biol Engn, Tianjin 300072, Peoples R China

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2015年 / 177卷 / 07期

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划); 美国国家科学基金会;

关键词：

Rhizopus oryzae; Fumaric acid; Acidic stress; Metabolic profile; SACCHAROMYCES-CEREVISIAE; OSMOTIC-STRESS; TOLERANCE; ADAPTATION; GLYCEROL;

D O I：

10.1007/s12010-015-1831-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ensuring a suitable pH is a major problem in industrial organic acid fermentation. To circumvent this problem, we used a metabolic profiling approach to analyze metabolite changes in Rhizopus oryzae under different pH conditions. A correlation between fumaric acid production and intracellular metabolic characteristics of R. oryzae was revealed by principal component analysis. The results showed that to help cell survival in the presence of low pH, R. oryzae altered amino acid and fatty acid metabolism and promoted sugar or sugar alcohol synthesis, corresponding with a suppressing of energy metabolism, phenylalanine, and tyrosine synthesis and finally resulting in the low performance of fumaric acid production. Based on this observation, 1 % linoleic acid was added to the culture medium in pH 3.0 to decrease the carbon demand for cell survival, and the fumaric acid titer was enhanced by 39.7 % compared with the control (pH 3.0 without linoleic acid addition), reaching 18.3 g/L after 84 h of fermentation. These findings provide new insights into the mechanism by which R. oryzae responds to acidic stress and would be helpful for the development of efficient strategies for fumaric acid production at low pH.

引用

页码：1508 / 1519

页数：12

共 21 条

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