Fluorescence Lifetime Imaging Microscopy (FLIM) of Quantum Dots in Living Cells

被引：2

作者：

Nadeau, Jay ^{[1
]}

Carlini, Lina ^{[1
]}

机构：

[1] McGill Univ, Dept Biomed Engn, Montreal, PQ, Canada

来源：

COLLOIDAL NANOCRYSTALS FOR BIOMEDICAL APPLICATIONS VIII | 2013年 / 8595卷

关键词：

Quantum Dots; InP; CdSe; Fluorescence Lifetime Imaging; Lifetime; Decay; BIOSENSORS; CDSE;

D O I：

10.1117/12.2013379

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

Fluorescence lifetime imaging microscopy (FLIM) is an emerging imaging technique that can indicate environmental factors such as pH and redox potential by the effect of these factors on the fluorescence lifetimes of fluorophores. Semiconductor quantum dots (QDs) are highly sensitive to environment and so are ideal for use in FLIM, although certain experimental parameters must be carefully considered for QD imaging to account for their long lifetimes and two-photon behavior. We image the uptake of three types of QDs in cultured fibroblasts and show some preliminary results on the effects of endosomes and lysosomes on QD lifetimes. These results indicate the feasibility of FLIM for studies using QDs in live cells.

引用

页数：10

共 21 条

[1] The use of heat transfer fluids in the synthesis of high-quality CdSe quantum dots, core/shell quantum dots, and quantum rods [J].