Production, characterization and control of a Neisseria meningitidis hexavalent class 1 outer membrane protein containing vesicle vaccine

被引：133
作者：
Claassen, I
Meylis, J
vanderLey, P
Peeters, C
Brons, H
Robert, J
Borsboom, D
vanderArk, A
vanStraaten, I
Roholl, P
Kuipers, B
Poolman, J
机构：
[1] NATL INST PUBL HLTH & ENVIRONM PROTECT,LAB VACCINE DEV & IMMUNE MECH,NL-3720 BA BILTHOVEN,NETHERLANDS
[2] NATL INST PUBL HLTH & ENVIRONM PROTECT,LAB PROD BACTERIAL VACCINES,NL-3720 BA BILTHOVEN,NETHERLANDS
[3] NATL INST PUBL HLTH & ENVIRONM PROTECT,PATHOL LAB,NL-3720 BA BILTHOVEN,NETHERLANDS
来源：
VACCINE | 1996年 / 14卷 / 10期
关键词：
D O I：
10.1016/0264-410X(96)00020-5
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
An experimental serogroup B meningococcal vaccine was prepared from two genetically engineered strains; each expressing three different class 1 outer membrane proteins (OMPs) (PorA). The two strains expressed the subtypes P1.7,16,P1.5,2;P1.19,15 and P1.5(c),10;P1.12,13;P1.7(h),4, respectively. Outer membrane vesicles (OMV) were prepared from these strains by deoxycholate extraction, mixed with aluminiumphosphate as adjuvant and formulated to final vaccines. The class 1 OMPs represent ca 90% of the protein in the vaccine. The vaccine was found safe for human use and induced a bactericidal immune response in mice against five of the six wild type strains, which served as donors for the various por A genes. Copyright (C) 1996 Elsevier Science Ltd.
引用
收藏
页码：1001 / 1008
页数：8
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