Properties of the Bacillus pumilus Glutamyl endopeptidase at different growth stages of its recombinant strain

被引：1
|
作者：
Balaban, N. P. [1 ]
Danilova, Yu V. [1 ]
Shamsutdinov, T. R. [1 ]
Mardanova, A. M. [1 ]
Cheremin, A. M. [1 ]
Rudenskaya, G. N. [2 ]
Sharipova, M. R. [1 ]
机构：
[1] Kazan Fed Univ, Kazan 420008, Russia
[2] Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119991, Russia
来源：
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2013年 / 39卷 / 01期
关键词：
glutamyl endopeptidase; B; pumilus; heterologous protein; isolation; different phases of the culture growth; ACID-SPECIFIC ENDOPEPTIDASE; MOLECULAR-CLONING; INTERMEDIUS; SUBTILIS; LICHENIFORMIS; PURIFICATION; SEQUENCE; PROTEINASE; PROTEASE; BINDING;
D O I：
10.1134/S1068162013010032
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Bacillus pumilus 3-19 Glutamyl peptidase (EC 3.4.21.19) was isolated from the culture medium of the B. subtilis recombinant strain at the following stages of the bacillus growth: a decelerating growth phase and a stationary growth phase. The action of the purified preparations of the enzyme on different phases of its growth was studied on the insulin B-chain and various protein and peptide substrates. Physicochemical properties of the enzyme were compared for different phases of its growth. The glutamyl endopeptidase preparations differed in their catalytic characteristics and their sensitivity to the metal cations.
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收藏
页码：40 / 47
页数：8
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