The anti-diabetic AMPK activator AICAR reduces IL-6 and IL-8 in human adipose tissue and skeletal muscle cells

被引：57
作者：
Lihn, Aina S. [1 ]
Pedersen, Steen B. [1 ]
Lund, Sten [2 ]
Richelsen, Bjorn [1 ]
机构：
[1] Aarhus Univ Hosp, Dept Endocrinol & Metab, Aarhus Sygehus, DK-8000 Aarhus C, Denmark
[2] Aarhus Univ Hosp, Aarhus Sygehus, Med Dept Endocrinol & Diabet M, DK-8000 Aarhus C, Denmark
来源：
MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2008年 / 292卷 / 1-2期
基金：
英国医学研究理事会;
关键词：
AICAR; IL-6; IL-8; GLUT4; AMPK; human adipose tissue; human skeletal muscle; rat skeletal muscle;
D O I：
10.1016/j.mce.2008.06.004
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Increased production of inflammatory cytokines is suggested to be of importance for initiation and progression of insulin resistance. Chronic treatment with the AMP analogue 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleoside (AICAR) has been shown to improve insulin sensitivity and prevent the development of diabetes in rodents. We investigated the effects of AICAR on the expression and production of inflammatory cytokines from human adipose tissue and skeletal muscle cells as well as intact rat skeletal muscles in vitro. AICAR dose-dependently decreased interleukin-6 (IL-6) gene expression and secretion in human adipose tissue and in human skeletal muscle cells. In parallel, AICAR inhibited interleukin-8 (IL-8) secretion in human adipose tissue and skeletal muscle cells, as well as reduced IL-8 gene expression in skeletal muscle cells. In intact rat extensor digitorum longus (EDL) muscle fibres AICAR markedly decreased IL-6 and IL-8 gene expression. In conclusion, AICAR inhibits the production of IL-6 and IL-8 human adipose tissue and in skeletal muscle cells. We suggest that decreased cytokine production might play a role for the AICAR-induced increase in insulin sensitivity. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：36 / 41
页数：6
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