Protein kinase C enables the regulatory circuit that connects membrane synthesis to ribosome synthesis in Saccharomyces cerevisiae

被引:77
作者
Nierras, CR [1 ]
Warner, JR [1 ]
机构
[1] Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA
关键词
D O I
10.1074/jbc.274.19.13235
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The balanced growth of a cell requires the integration of major systems such as DNA replication, membrane biosynthesis, and ribosome formation. An example of such integration is evident from our recent finding that, in Saccharomyces cerevisiae, any failure in the secretory pathway leads to severe repression of transcription of both rRNA and ribosomal protein genes. We have attempted to determine the regulatory circuit(s) that connects the secretory pathway with the transcription of ribosomal genes. Experiments show that repression does not occur through the circuit that responds to misfolded proteins in the endoplasmic reticulum, nor does it occur through circuits known to regulate ribosome synthesis, e.g. the stringent response, or the cAMP pathway. Rather, it appears to depend on a stress response at the plasma membrane that is transduced through protein kinase C (PKC), Deletion of PKC1 relieves the repression of both ribosomal protein and rRNA genes that occurs in response to a defect in the secretory pathway. We propose that failure of the secretory pathway prevents the synthesis of new plasma membrane. As protein synthesis continues, stress develops in the plasma membrane. This stress is monitored by Pkc1p, which initiates a signal transduction pathway that leads to repression of transcription of the rRNA and ribosomal protein genes. The importance of the transcription of the 137 ribosomal protein genes to the economy of the cell is apparent from the existence of at least three distinct pathways that can effect the repression of this set of genes.
引用
收藏
页码:13235 / 13241
页数:7
相关论文
共 79 条
[1]   CDC42 AND CDC43, 2 ADDITIONAL GENES INVOLVED IN BUDDING AND THE ESTABLISHMENT OF CELL POLARITY IN THE YEAST SACCHAROMYCES-CEREVISIAE [J].
ADAMS, AEM ;
JOHNSON, DI ;
LONGNECKER, RM ;
SLOAT, BF ;
PRINGLE, JR .
JOURNAL OF CELL BIOLOGY, 1990, 111 (01) :131-142
[2]   POSITIONING OF CELL-GROWTH AND DIVISION AFTER OSMOTIC-STRESS REQUIRES A MAP KINASE PATHWAY [J].
BREWSTER, JL ;
GUSTIN, MC .
YEAST, 1994, 10 (04) :425-439
[3]   AN OSMOSENSING SIGNAL TRANSDUCTION PATHWAY IN YEAST [J].
BREWSTER, JL ;
DEVALOIR, T ;
DWYER, ND ;
WINTER, E ;
GUSTIN, MC .
SCIENCE, 1993, 259 (5102) :1760-1763
[4]   RAS GENES IN SACCHAROMYCES-CEREVISIAE - SIGNAL TRANSDUCTION IN SEARCH OF A PATHWAY [J].
BROACH, JR .
TRENDS IN GENETICS, 1991, 7 (01) :28-33
[5]   CAMP-INDEPENDENT CONTROL OF SPORULATION, GLYCOGEN-METABOLISM, AND HEAT-SHOCK RESISTANCE IN S-CEREVISIAE [J].
CAMERON, S ;
LEVIN, L ;
ZOLLER, M ;
WIGLER, M .
CELL, 1988, 53 (04) :555-566
[6]   TRANSCRIPTIONAL INDUCTION OF GENES ENCODING ENDOPLASMIC-RETICULUM RESIDENT PROTEINS REQUIRES A TRANSMEMBRANE PROTEIN-KINASE [J].
COX, JS ;
SHAMU, CE ;
WALTER, P .
CELL, 1993, 73 (06) :1197-1206
[7]   A novel mechanism for regulating activity of a transcription factor that controls the unfolded protein response [J].
Cox, JS ;
Walter, P .
CELL, 1996, 87 (03) :391-404
[8]  
DAVENPORT KR, 1995, J BIOL CHEM, V270, P30157
[9]   Exploring the metabolic and genetic control of gene expression on a genomic scale [J].
DeRisi, JL ;
Iyer, VR ;
Brown, PO .
SCIENCE, 1997, 278 (5338) :680-686
[10]   Cluster analysis and display of genome-wide expression patterns [J].
Eisen, MB ;
Spellman, PT ;
Brown, PO ;
Botstein, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) :14863-14868