Tumor Lysing Genetically Engineered T Cells Loaded with Multi-Modal Imaging Agents

被引:26
|
作者
Bhatnagar, Parijat [1 ]
Alauddin, Mian [2 ]
Bankson, James A. [3 ]
Kirui, Dickson [4 ]
Seifi, Payam [5 ]
Huls, Helen [6 ]
Lee, Dean A. [6 ,7 ]
Babakhani, Aydin [5 ]
Ferrari, Mauro [4 ]
Li, King C. [8 ]
Cooper, Laurence J. N. [6 ,7 ]
机构
[1] Baylor Coll Med, Dept Obstet & Gynecol, Houston, TX 77030 USA
[2] Univ Texas MD Anderson Canc Ctr, Div Diagnost Imaging, Dept Expt Diagnost Imaging, Houston, TX 77030 USA
[3] Univ Texas MD Anderson Canc Ctr, Div Diagnost Imaging, Dept Imaging Phys, Houston, TX 77030 USA
[4] Methodist Hosp, Res Inst, Dept Nanomed, Houston, TX 77030 USA
[5] Rice Univ, Dept Elect & Comp Engn, Houston, TX 77005 USA
[6] Univ Texas MD Anderson Canc Ctr, Div Pediat, Houston, TX 77030 USA
[7] Univ Texas MD Anderson Canc Ctr, Dept Immunol, Houston, TX 77030 USA
[8] Methodist Hosp, Res Inst, Dept Translat Imaging, Houston, TX 77030 USA
来源
SCIENTIFIC REPORTS | 2014年 / 4卷
关键词
TRACKING; NANOPARTICLES; TRAFFICKING;
D O I
10.1038/srep04502
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Genetically-modified T cells expressing chimeric antigen receptors (CAR) exert anti-tumor effect by identifying tumor-associated antigen (TAA), independent of major histocompatibility complex. For maximal efficacy and safety of adoptively transferred cells, imaging their biodistribution is critical. This will determine if cells home to the tumor and assist in moderating cell dose. Here, T cells are modified to express CAR. An efficient, non-toxic process with potential for cGMP compliance is developed for loading high cell number with multi-modal (PET-MRI) contrast agents (Super Paramagnetic Iron Oxide Nanoparticles - Copper-64; SPION-Cu-64). This can now be potentially used for Cu-64-based whole-body PET to detect T cell accumulation region with high-sensitivity, followed by SPION-based MRI of these regions for high-resolution anatomically correlated images of T cells. CD19-specific-CAR1SPION(pos) T cells effectively target in vitro CD191 lymphoma.
引用
收藏
页数:6
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