LC-MS-MS Determination of Rotenone, Deguelin, and Rotenolone in Human Serum

被引：17

作者：

Caboni, Pierluigi ^{[1
]}

Sarais, Giorgia ^{[1
]}

Vargiu, Simona ^{[1
]}

De Luca, Maria A. ^{[1
]}

Garau, Vincenzo L. ^{[1
]}

Ibba, Antonio ^{[2
]}

Cabras, Paolo ^{[1
]}

机构：

[1] Univ Cagliari, Dept Toxicol, I-09124 Cagliari, Italy

[2] Policlin Univ, Occupat Hlth Sect, Dept Publ Hlth, I-09042 Cagliari, Italy

来源：

CHROMATOGRAPHIA | 2008年 / 68卷 / 9-10期

关键词：

Column liquid chromatography-tandem mass spectrometry; Rotenone; Deguelin; Rotenolone; Human serum;

D O I：

10.1365/s10337-008-0830-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

For the first time we report a rapid and sensitive LC-MS-MS method for quantification of rotenone, deguelin, and rotenolone in human serum. The analytical procedure involves extraction with ethyl acetate without further clean-up. The active ingredients were separated on a C-8 reversed-phase column by isocratic elution. Eleven simultaneous transitions of precursor ions were monitored. Excellent selectivity and sensitivity enables quantification and identification of low levels of rotenoids (LOD 2 ng mL(-1), LOQ 5 ng mL(-1)) in human serum.

引用

页码：739 / 745

页数：7

共 20 条

[1] Quantification of cidofovir in human serum by LC-MS/MS for children [J].

Breddernann, Andre ;

Hsien, Linda ;

Tot, Edith ;

Laeer, Stephanie .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2008, 861 (01) :1-9

[2] Fast and versatile multiresidue method for the analysis of botanical insecticides on fruits and vegetables by HPLC/DAD/MS [J].

Caboni, P ;

Sarais, G ;

Angioni, A ;

Garau, VL ;

Cabras, P .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (22) :8644-8649

[3] Rotenone, deguelin, their metabolites, and the rat model of Parkinson's disease [J].

Caboni, P ;

Sherer, TB ;

Zhang, NJ ;

Taylor, G ;

Na, HM ;

Greenamyre, JT ;

Casida, JE .

CHEMICAL RESEARCH IN TOXICOLOGY, 2004, 17 (11) :1540-1548

[4] High resolution electrospray and electrospray tandem mass spectra of rotenone and its isoxazoline cycloadducts [J].

Cordaro, M ;

Di Donna, L ;

Grassi, G ;

Maiuolo, L ;

Mazzotti, F ;

Perri, E ;

Sindona, G ;

Tagarelli, A .

EUROPEAN JOURNAL OF MASS SPECTROMETRY, 2004, 10 (05) :691-697

[5]

DEWILDE AR, 1986, J FORENSIC SCI, V31, P1492

[6] Assay of rotenone in river water by high-throughput tandem mass spectrometry and multiple-reaction monitoring methodology [J].

Di Donna, L ;

Mazzotti, F ;

Sindona, G ;

Tagarelli, A .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2005, 19 (12) :1575-1577

[7] Anticancer action of cube insecticide:: Correlation for rotenoid constituents between inhibition of NADH:ubiquinone oxidoreductase and induced ornithine decarboxylase activities [J].

Fang, NB ;

Casida, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (07) :3380-3384

[8] Cube resin insecticide: Identification and biological activity of 29 rotenoid constituents [J].

Fang, NB ;

Casida, JE .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (05) :2130-2136

[9]

FUKAMI H, 1971, NATURALLY OCCURRING, P71

[10]

Gosselin R. E., 1984, CLIN TOXICOLOGY COMM, P111

← 1 2 →