BNIP3 Upregulation Characterizes Cancer Cell Subpopulation With Increased Fitness and Proliferation

被引：7

作者：

Zhu, Yanyan ^{[1
]}

Chen, Bowang ^{[2
,3
]}

Yan, Junya ^{[1
]}

Zhao, Wendi ^{[4
]}

Dou, Pengli ^{[5
]}

Sun, Na ^{[2
]}

Wang, Yaokai ^{[6
]}

Huang, Xiaoyun ^{[2
,3
]}

机构：

[1] Zhengzhou Univ, Peoples Hosp, Henan Prov Peoples Hosp, Sch Clin Med,Dept Oncol, Zhengzhou, Peoples R China

[2] Intelliphecy, Dept Computat Oncol, Shenzhen, Peoples R China

[3] Intelliphecy, Ctr Syst Biol, Shenzhen, Peoples R China

[4] Henan Univ, Henan Prov Peoples Hosp, Sch Clin Med, Dept Oncol, Zhengzhou, Peoples R China

[5] Intelliphecy, Dept Expt Canc Modeling, Nanjing, Peoples R China

[6] Univ Hong Kong, Shenzhen Hosp, Dept Gynecol & Obstet, Shenzhen, Peoples R China

来源：

FRONTIERS IN ONCOLOGY | 2022年 / 12卷

基金：

美国国家科学基金会;

关键词：

BNIP3; ScRNA-seq; mitophagy; systems biology; cancer heterogeneity; SINGLE-CELL; BREAST-CANCER; HYPOXIA; AUTOPHAGY; PROGRESSION; MITOPHAGY; CARCINOMA; DYNAMICS; GENE;

D O I：

10.3389/fonc.2022.923890

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

BNIP3 is a BH3-only protein with both pro-apoptotic and pro-survival roles depending on the cellular context. It remains unclear how BNIP3 RNA level dictates cell fate decisions of cancer cells. Here, we undertook a quantitative analysis of BNIP3 expression and functions in single-cell datasets of various epithelial malignancies. Our results demonstrated that BNIP3 upregulation characterizes cancer cell subpopulations with increased fitness and proliferation. We further validated the upregulation of BNIP3 in liver cancer 3D organoid cultures compared with 2D culture. Taken together, the combination of in silico perturbations using public single-cell datasets and experimental cancer modeling using organoids ushered in a new approach to address cancer heterogeneity.

引用

页数：9

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