A novel approach for quantitation of glucosylceramide in human dried blood spot using LC-MS/MS

被引：10

作者：

Ji, Allena Ji ^{[1
]}

Wang, Haixing ^{[1
]}

Ziso-Qejvanaj, Enida ^{[1
]}

Zheng, Kefei ^{[1
]}

Chung, Lee Lee ^{[1
]}

Foley, Timothy ^{[1
]}

Chuang, Wei-Lien ^{[2
]}

Richards, Susan ^{[1
]}

Sung, Crystal ^{[1
]}

机构：

[1] Sanofi, Clin Specialty Lab, DSAR, Framingham, MA 01701 USA

[2] Sanofi, Biol Mass Spectrometry & Biomarker Res, Framingham, MA 01701 USA

来源：

BIOANALYSIS | 2015年 / 7卷 / 12期

关键词：

GAUCHER-DISEASE; BIOANALYTICAL METHODS; CERAMIDE TRIHEXOSIDE; HUMAN PLASMA; ASSAY BIAS; SPHINGOLIPIDS; HEMATOCRIT; QUANTIFICATION; IMPLEMENTATION; SPHINGOMYELIN;

D O I：

10.4155/bio.15.77

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Glucosylceramide, an efficacy biomarker for Gaucher Type 1 disease, exhibits poor solubility in polar solvents and whole blood which makes it difficult to prepare a homogenous blood standard. Results: We developed a novel method using standard addition approach by spiking a small volume of analyte solution on the surface of prespotted dried blood spot. The whole spots were punched out for subsequent extraction and LC-MS/MS analysis. The assay performance met all validation acceptance criteria. Glucosylceramide concentrations in 50 paired plasma and dry blood spot samples obtained from Gaucher Type 1 patients were tested and the results demonstrated the feasibility of using the DBS method for clinical biomarker monitoring. Conclusion: The new approach greatly improves assay precision and accuracy.

引用

页码：1483 / 1496

页数：14

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