A novel approach for quantitation of glucosylceramide in human dried blood spot using LC-MS/MS

被引：10

作者：

Ji, Allena Ji ^{[1
]}

Wang, Haixing ^{[1
]}

Ziso-Qejvanaj, Enida ^{[1
]}

Zheng, Kefei ^{[1
]}

Chung, Lee Lee ^{[1
]}

Foley, Timothy ^{[1
]}

Chuang, Wei-Lien ^{[2
]}

Richards, Susan ^{[1
]}

Sung, Crystal ^{[1
]}

机构：

[1] Sanofi, Clin Specialty Lab, DSAR, Framingham, MA 01701 USA

[2] Sanofi, Biol Mass Spectrometry & Biomarker Res, Framingham, MA 01701 USA

来源：

BIOANALYSIS | 2015年 / 7卷 / 12期

关键词：

GAUCHER-DISEASE; BIOANALYTICAL METHODS; CERAMIDE TRIHEXOSIDE; HUMAN PLASMA; ASSAY BIAS; SPHINGOLIPIDS; HEMATOCRIT; QUANTIFICATION; IMPLEMENTATION; SPHINGOMYELIN;

D O I：

10.4155/bio.15.77

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Glucosylceramide, an efficacy biomarker for Gaucher Type 1 disease, exhibits poor solubility in polar solvents and whole blood which makes it difficult to prepare a homogenous blood standard. Results: We developed a novel method using standard addition approach by spiking a small volume of analyte solution on the surface of prespotted dried blood spot. The whole spots were punched out for subsequent extraction and LC-MS/MS analysis. The assay performance met all validation acceptance criteria. Glucosylceramide concentrations in 50 paired plasma and dry blood spot samples obtained from Gaucher Type 1 patients were tested and the results demonstrated the feasibility of using the DBS method for clinical biomarker monitoring. Conclusion: The new approach greatly improves assay precision and accuracy.

引用

页码：1483 / 1496

页数：14

共 27 条

[1]

Adam BW, 2000, CLIN CHEM, V46, P126

[2] Workshop Report: Crystal City V-Quantitative Bioanalytical Method Validation and Implementation: The 2013 Revised FDA Guidance [J].

Booth, Brian ;

Arnold, Mark E. ;

DeSilva, Binodh ;

Amaravadi, Lakshmi ;

Dudal, Sherri ;

Fluhler, Eric ;

Gorovits, Boris ;

Haidar, Sam H. ;

Kadavil, John ;

Lowes, Steve ;

Nicholson, Robert ;

Rock, Marie ;

Skelly, Michael ;

Stevenson, Lauren ;

Subramaniam, Sriram ;

Weiner, Russell ;

Woolf, Eric .

AAPS JOURNAL, 2015, 17 (02) :277-288

[3] Analysis of sphingolipids in extracted human plasma using liquid chromatography electrospray ionization tandem mass spectrometry [J].

Bui, Hai H. ;

Leohr, Jennifer K. ;

Kuo, Ming-Shang .

ANALYTICAL BIOCHEMISTRY, 2012, 423 (02) :187-194

[4] An Introduction to Sphingolipid Metabolism and Analysis by New Technologies [J].

Chen, Yanfeng ;

Liu, Ying ;

Sullards, M. Cameron ;

Merrill, Alfred H., Jr. .

NEUROMOLECULAR MEDICINE, 2010, 12 (04) :306-319

[5] Lyso-sphingomyelin is elevated in dried blood spots of Niemann-Pick B patients [J].

Chuang, Wei-Lien ;

Pacheco, Joshua ;

Cooper, Samantha ;

McGovern, Margaret M. ;

Cox, Gerald F. ;

Keutzer, Joan ;

Zhang, X. Kate .

MOLECULAR GENETICS AND METABOLISM, 2014, 111 (02) :209-211

[6] The effect of hematocrit on assay bias when using DBS samples for the quantitative bioanalysis of drugs [J].

Denniff, Philip ;

Spooner, Neil .

BIOANALYSIS, 2010, 2 (08) :1385-1395

[7]

Fauci AS, 2008, HARRISONSS PRINCIPLE

[8] Quantification of glucosylceramide in plasma of Gaucher disease patients [J].

Gomes Muller, Maria Viviane ;

Petry, Andre ;

Vianna, Luciene Pinheiro ;

Breier, Ana Carolina ;

Michelin-Tirelli, Kristiane ;

Pires, Ricardo Flores ;

Treis Trindade, Vera Maria ;

Coelho, Janice Carneiro .

BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2010, 46 (04) :643-649

[9] HPLC for simultaneous quantification of total ceramide, glucosylceramide, and ceramide trihexoside concentrations in plasma [J].

Groener, Johanna E. M. ;

Poorthuis, Ben J. H. M. ;

Kuiper, Sijmen ;

Helmond, Mariette T. J. ;

Hollak, Carla E. M. ;

Aerts, Johannes M. F. G. .

CLINICAL CHEMISTRY, 2007, 53 (04) :742-747

[10] An Improved Method for Analysis of Glucosylceramide Species Having cis-8 and trans-8 Isomers of Sphingoid Bases by LC-MS/MS [J].

Imai, Hiroyuki ;

Hattori, Hideyasu ;

Watanabe, Masayuki .

LIPIDS, 2012, 47 (12) :1221-1229

← 1 2 3 →