The Effects of α-Lipoic Acid against Testicular Ischemia-Reperfusion Injury in Rats

被引：43

作者：

Ozbal, Seda ^{[1
]}

Ergur, Bekir Ugur ^{[1
]}

Erbil, Guven ^{[1
]}

Tekmen, Isil ^{[1
]}

Bagriyanik, Alper ^{[1
]}

Cavdar, Zahide ^{[2
]}

机构：

[1] Dokuz Eylul Univ Inciralti, Dept Histol & Embryol, Sch Med, TR-35340 Izmir, Turkey

[2] Dokuz Eylul Univ, Dept Mol Med, Hlth Sci Inst, Sch Med, TR-35340 Izmir, Turkey

来源：

SCIENTIFIC WORLD JOURNAL | 2012年

关键词：

NITRIC-OXIDE SYNTHASE; ISCHEMIA/REPERFUSION INJURY; GROWTH-FACTOR; TORSION; ANTIOXIDANT; EXPRESSION; APOPTOSIS; PROTECTS; STRESS;

D O I：

10.1100/2012/489248

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Testicular torsion is one of the urologic emergencies occurring frequently in neonatal and adolescent period. Testis is sensitive to ischemia-reperfusion injury, and, therefore, ischemia and consecutive reperfusion cause an enhanced formation of reactive oxygen species that result in testicular cell damage and apoptosis. alpha-lipoic acid is a free radical scavenger and a biological antioxidant. It is widely used in the prevention of oxidative stress and cellular damage. We aimed to investigate the protective effect of alpha-lipoic acid on testicular damage in rats subjected to testicular ischemia-reperfusion injury. 35 rats were randomly divided into 5 groups: control, sham operated, ischemia, ischemia-reperfusion, and ischemia-reperfusion +lipoic acid groups, 2 h torsion and 2 h detorsion of the testis were performed. Testicular cell damage was examined by H-E staining. TUNEL and active caspase-3 immunostaining were used to detect germ cell apoptosis. GPx, SOD activity, and MDA levels were evaluated. Histological evaluation showed that alpha-lipoic acid pretreatment reduced testicular cell damage and decreased TUNEL and caspase-3-positive cells. Additionally, alpha-lipoic acid administration decreased the GPx and SOD activity and increased the MDA levels. The present results suggest that LA is a potentially beneficial agent in protecting testicular I/R in rats.

引用

页数：8

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