Genetic diversity of the genus Ostreopsis Schmidt: phylogeographical considerations and molecular methodology applications for field detection in the Mediterranean Sea

被引：37

作者：

Penna, Antonella ^{[1
]}

Fraga, Santiago ^{[2
]}

Battocchi, Cecilia ^{[1
]}

Casabianca, Silvia ^{[1
]}

Perini, Federico ^{[1
]}

Capellacci, Samuela ^{[1
]}

Casabianca, Anna ^{[1
]}

Riobo, Pilar ^{[3
]}

Giacobbe, Maria Grazia ^{[4
]}

Totti, Cecilia ^{[5
]}

Accoroni, Stefano ^{[5
]}

Vila, Magda ^{[6
]}

Rene, Albert ^{[6
]}

Scardi, Michele ^{[7
]}

Aligizaki, Katerina ^{[8
]}

Lam Nguyen-Ngoc ^{[9
]}

Vernesi, Cristiano ^{[10
]}

机构：

[1] Univ Urbino, Dep Biomol Sci, I-61100 Pesaro, Italy

[2] IEO, Vigo 36390, Spain

[3] CSIC, IIM, Vigo 36208, Spain

[4] CNR, Ist Marine & Coastal Environm IAMC, I-98122 Messina, Italy

[5] Univ Politecn Marche, Dip Sci Vita & Ambiente, I-60131 Ancona, Italy

[6] CSIC, ICM, E-08003 Barcelona, Spain

[7] Univ Roma Tor Vergata, Dept Biol, I-00133 Rome, Italy

[8] Aristotle Univ Thessaloniki, Dep Biol, Thessaloniki 54124, Greece

[9] Dept Marine Plankton Inst Oceanog, Nhatrang City, Vietnam

[10] Fdn Edmund Mach, Dep Biodivers & Mol Ecol, I-38040 Viote Del Monte Bondone, Trento, Italy

来源：

CRYPTOGAMIE ALGOLOGIE | 2012年 / 33卷 / 02期

关键词：

Benthic dinoflagellate; distribution; Mediterranean Sea; Ostreopsis; ribosomal genes; qrt-PCR; REAL-TIME PCR; COOLIA DINOPHYCEAE; PALYTOXIN; QUANTIFICATION; OUTBREAK; COASTS; STATE; ASSAY;

D O I：

10.7872/crya.v33.iss2.2011.153

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

This study reports some recent phylogeographical considerations on the genus Ostreopsis distribution worldwide, with particular attention to the Mediterranean Sea, and new recent advances on the quali-quantitiative detection of Ostreopsis species along coastal areas of the Mediterranean Sea based on the PCR and quantitative real time PCR (qrt-PCR) assays. It was found that O. cf. ovata is widely dispersed throughout tropical and warm temperate coastal areas. In the Atlantic/Mediterranean region it represents a panmictic population that is highly divergent from Indo-Pacific populations. Furthermore, we demonstrated that the developed qrt-PCR assay is specific, robust and high sample throughput for the quantification of the toxic O. cf. ovata in the environmental samples. This molecular approach may be considered alternative to traditional methods of microscopy and applied for the survey of benthic toxic microalgal species in marine ecosystems.

引用

页码：153 / 163

页数：11

共 22 条

[1] Ostreopsis cf. ovata bloom in the northern Adriatic Sea during summer 2009: Ecology, molecular characterization and toxin profile [J].