Bacteria-induced IMD-Relish-AMPs pathway activation in Chinese mitten crab

被引:24
作者
Bai, Longwei [1 ]
Zhou, Kaimin [1 ]
Li, Hao [1 ]
Qin, Yukai [1 ]
Wang, Qun [1 ]
Li, Weiwei [1 ]
机构
[1] East China Normal Univ, Sch Life Sci, Lab Invertebrate Immunol Def & Reprod Biol, Shanghai 200241, Peoples R China
基金
中国国家自然科学基金;
关键词
Bacteria-induced; IMD pathway; Nuclear translocation; Antimicrobial peptides; Eriocheir sinensis; IMMUNE-RESPONSE; LITOPENAEUS-VANNAMEI; FUNCTIONAL-ANALYSIS; PGRP-LC; DROSOPHILA; RECOGNITION; GENE; SHRIMP; EXPRESSION; RECEPTOR;
D O I
10.1016/j.fsi.2020.08.046
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
The innate immune response is an important line of defense against invading pathogens in invertebrates. Signaling pathways, including the IMD pathway, play critical roles in the production of antimicrobial peptides (AMPs), which induce the transcription of immune effectors that protect against bacterial invasion. In the present study, the cDNA of IMD from Eriocheir sinensis was cloned (designated EsIMD) and shown to be significantly upregulated following Gram-positive and Gram-negative bacterial infection. In vivo and in vitro studies collectively suggested that both the Gram-negative bacterium Vibrio parahemolyticus and the Gram-positive bacteria Staphylococcus aureus and Bacillus subtilis elicit the translocation of Relish. Moreover, EsIMD positively regulated EsRelish translocation from the cytoplasm to the nucleus following stimulation with both Gram-positive and Gram-negative bacteria. EsRelish knockdown in hemocytes significantly suppressed AMPs' expression. Furthermore, both Lys-type and DAP-type peptidoglycan-containing bacteria activated the IMD pathway and elicited antibacterial responses in crab. Conclusively, these findings demonstrate that both Gram-positive and Gram-negative bacteria activate IMD signaling, via a mechanism that is distinct with that by which Gramnegative bacteria activate IMD signaling in Drosophila. These findings might pave the way for a better understanding of the innate immune system and the fundamental network of the IMD signaling pathway in crustacean.
引用
收藏
页码:866 / 875
页数:10
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