Immunosensors for pesticide analysis: Antibody production and sensor development

被引：47

作者：

Suri, CR ^{[1
]}

Raje, M ^{[1
]}

Varshney, GC ^{[1
]}

机构：

[1] Inst Microbial Technol, Chandigarh 160036, India

来源：

CRITICAL REVIEWS IN BIOTECHNOLOGY | 2002年 / 22卷 / 01期

关键词：

immunosensor; antibody; immune-complex; pesticides;

D O I：

10.1080/07388550290789441

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Immunosensors, a type of affinity biosensor, are based on the binding interactions between an immobilized biomolecule (antibody/antigen) on the electronic transducer surface with the analyte of interest (antigen/antibody), resulting in a detectable signal. The sensor system takes advantage of the high selectivity provided by the molecular recognition characteristic of an antibody, which binds reversibly with a specific antigen. This review article presents the current status of immunosensors, highlighting their potential benefits and limitations for pesticide analysis. The basic criteria for generating specific antibodies against low-molecular-mass pesticides, which are usually nonimmunogenic in nature, are briefly discussed. The article also describes the fundamentals of important transducer technologies and their use in inummosensor development.

引用

页码：15 / 32

页数：18

共 73 条

[1] Determination of organophosphorus and carbamate pesticides using a piezoelectric biosensor [J].