A 286 bp upstream regulatory region of a rice anther-specific gene, OSIPP3, confers pollen-specific expression in Arabidopsis

被引：10
作者：
Khurana, Reema [1 ]
Kathuria, Hitesh [2 ]
Mukhopadhyay, Arnab [3 ]
Kapoor, Sanjay [1 ]
Tyagi, Akhilesh K. [1 ,4 ]
机构：
[1] Univ Delhi S Campus, Dept Plant Mol Biol, Interdisciplinary Ctr Plant Genom, New Delhi 110021, India
[2] Indiana Univ E, Sch Nat Sci & Math, Richmond, IN 47374 USA
[3] Natl Inst Immunol, New Delhi 110067, India
[4] Natl Inst Plant Genome Res, New Delhi 110067, India
来源：
BIOTECHNOLOGY LETTERS | 2013年 / 35卷 / 03期
关键词：
Arabidopsis; GUS; Pollen; Rice; Upstream regulatory region; PECTIN METHYLESTERASE; PROMOTER; ELEMENTS; PLANT; TRANSCRIPTION; INHIBITOR;
D O I：
10.1007/s10529-012-1100-7
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
OSIPP3 gene (coding for pectin methylesterase inhibitor protein) was isolated from a pre-pollinated inflorescence-specific cDNA library by differential screening of stage-specific libraries from Oryza sativa. OSIPP3 is present in the genome of rice as a single copy gene. OSIPP3 gene was expressed exclusively in the pre-pollinated spikelets of rice. Upstream regulatory region (URR) of OSIPP3 was isolated and a series of 5'-deletions were cloned upstream of GUS reporter gene and were used to transform Arabidopsis. OSIPP3_del1 and del2 transgenic plants showed GUS expression in root, anther and silique, while OSIPP3_del3 showed GUS activity only in anthers and siliques. Pollen-specific expression was observed in case of plants harboring OSIPP3_del4 construct. It can, therefore, be concluded that the OSIPP3 URR between -178 and +108 bp is necessary for conferring pollen-specific expression in Arabidopsis.
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页码：455 / 462
页数：8
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