The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification

被引：51

作者：

Abed, Mona ^{[1
]}

Verschueren, Erik ^{[2
]}

Budayeva, Hanna ^{[2
]}

Liu, Peter ^{[2
]}

Kirkpatrick, Donald S. ^{[2
]}

Reja, Rohit ^{[3
]}

Kummerfeld, Sarah K. ^{[3
,7
]}

Webster, Joshua D. ^{[4
]}

Gierke, Sarah ^{[4
]}

Reichelt, Mike ^{[4
]}

Anderson, Keith R. ^{[5
]}

Newman, Robert J. ^{[5
]}

Roose-Girma, Merone ^{[5
]}

Modrusan, Zora ^{[5
]}

Pektas, Hazal ^{[6
]}

Maltepe, Emin ^{[6
]}

Newton, Kim ^{[1
]}

Dixit, Vishva M. ^{[1
]}

机构：

[1] Genentech Inc, Physiol Chem Dept, San Francisco, CA 94080 USA

[2] Genentech Inc, Prot Chem Dept, San Francisco, CA USA

[3] Genentech Inc, Bioinformat & Computat Biol Dept, San Francisco, CA USA

[4] Genentech Inc, Pathol Dept, San Francisco, CA USA

[5] Genentech Inc, Mol Biol Dept, San Francisco, CA USA

[6] Univ Calif San Francisco, Div Neonatol, Ctr Reprod Sci, San Francisco, CA 94143 USA

[7] Garvin Inst Med Res, Kinghorn Ctr Clin Genom, Darlinghurst, NSW, Australia

来源：

PLOS ONE | 2019年 / 14卷 / 04期

关键词：

TRANSPOSABLE ELEMENTS; PACKAGING SIGNAL; GENOME; GENE; USP9X; RETROTRANSPOSON; UBIQUITINATION; EXPRESSION; STABILITY; ENVELOPE;

D O I：

10.1371/journal.pone.0214110

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Peg10 (paternally expressed gene 10) is an imprinted gene that is essential for placental development. It is thought to derive from a Ty3-gyspy LTR (long terminal repeat) retrotransposon and retains Gag and Pol-like domains. Here we show that the Gag domain of PEG10 can promote vesicle budding similar to the HIV p24 Gag protein. Expressed in a subset of mouse endocrine organs in addition to the placenta, PEG10 was identified as a substrate of the deubiquitinating enzyme USP9X. Consistent with PEG10 having a critical role in placental development, PEG10-deficient trophoblast stem cells (TSCs) exhibited impaired differentiation into placental lineages. PEG10 expressed in wild-type, differentiating TSCs was bound to many cellular RNAs including Hbegf (Heparin-binding EGF-like growth factor), which is known to play an important role in placentation. Expression of Hbegf was reduced in PEG10-deficient TSCs suggesting that PEG10 might bind to and stabilize RNAs that are critical for normal placental development.

引用

页数：18

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