Elucidation of possible molecular mechanisms underlying the estrogen-induced disruption of cartilage development in zebrafish larvae

被引:19
作者
He, Hanliang [1 ]
Wang, Chunqing [2 ]
Tang, Qifeng [3 ]
Yang, Fan [1 ]
Xu, Youjia [1 ]
机构
[1] Soochow Univ, Affiliated Hosp 2, Dept Orthopaed, Suzhou 215004, Peoples R China
[2] Guiyang Med Univ, Affiliated Hosp, Dept Orthopaed, Guiyang 550004, Peoples R China
[3] Benq Med Ctr Suzhou, Suzhou 215000, Peoples R China
基金
中国国家自然科学基金;
关键词
Estrogen; Zebrafish; Cartilage defect; RNA deep sequencing; Extracellular matrix; CELL-CYCLE CHECKPOINTS; EXTRACELLULAR-MATRIX; SIGNALING PATHWAYS; TGF-BETA; RECEPTOR; APOPTOSIS; WATER; MICE;
D O I
10.1016/j.toxlet.2018.02.023
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Estrogen can affect the cartilage development of zebrafish; however, the mechanism underlying its effects is not completely understood. Four-day-old zebrafish larvae were treated with 0.8 mu M estrogen, the 5 days post fertilization (dpf) zebrafish larvae did not demonstrate obvious abnormalities during development; however, the 6 dpf and 7 dpf larvae exhibited abnormal craniofacial bone development along with craniofacial bone degradation. RNA deep sequencing was performed to elucidate the mechanism involved. Gene Ontology functional and KEGG pathway enrichment analysis of differentially expressed genes (DEGs) showed that the extracellular matrix (ECM), extracellular region, ECM-interaction receptor, focal adhesion, cell cycle, apoptosis, and bone-related signaling pathways were disrupted. In these signaling pathways, the expressions of key genes, such as collagen encoded (col19a1a, col7a1, col7al, col18a1, and col9a3), MAPK signaling pathway (fgf19, fgf6a), TGF-beta signaling pathway (tgfbr1), and cell cycle (cdnk1a) genes were altered. The qRT-PCR results showed that after treatment with 0.8 mu M 17-ss estradiol (E2), col19a1a, col7a1, col7al, col18a1, col9a3, fgf6a, cdkn1a were downregulated, and fgf19, tgfr1 were upregulated, which were consistent with deep sequencing analysis. Therefore, the effect of estrogen on cartilage development might occur via multiple mechanisms. The study results demonstrate the mechanism underlying the effect of estrogen on cartilage development.
引用
收藏
页码:22 / 27
页数:6
相关论文
共 34 条
[11]   Environmental enrichment reduces the mnemonic and neural benefits of estrogen [J].
Gresack, JE ;
Frick, KM .
NEUROSCIENCE, 2004, 128 (03) :459-471
[12]   The multifaceted mechanisms of estradiol and estrogen receptor signaling [J].
Hall, JM ;
Couse, JF ;
Korach, KS .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (40) :36869-36872
[13]   Identification of Estrogen Target Genes during Zebrafish Embryonic Development through Transcriptomic Analysis [J].
Hao, Ruixin ;
Bondesson, Maria ;
Singh, Amar V. ;
Riu, Anne ;
McCollum, Catherine W. ;
Knudsen, Thomas B. ;
Gorelick, Daniel A. ;
Gustafsson, Jan-Ake .
PLOS ONE, 2013, 8 (11)
[14]  
Hofbauer L. C., 2004, Journal of Musculoskeletal & Neuronal Interactions, V4, P268
[15]   The Extracellular Matrix: Not Just Pretty Fibrils [J].
Hynes, Richard O. .
SCIENCE, 2009, 326 (5957) :1216-1219
[16]   Secretory COPII coat component Sec23a is essential for craniofacial chondrocyte maturation [J].
Lang, Michael R. ;
Lapierre, Lynne A. ;
Frotscher, Michael ;
Goldenring, James R. ;
Knapik, Ela W. .
NATURE GENETICS, 2006, 38 (10) :1198-1203
[17]   Estrogens and health in males [J].
Lombardi, G ;
Zarrilli, S ;
Colao, A ;
Paesano, L ;
Di Somma, C ;
Rossi, F ;
De Rosa, M .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2001, 178 (1-2) :51-55
[18]   CELLULAR STAGES IN CARTILAGE FORMATION AS REVEALED BY MORPHOMETRY, AUTORADIOGRAPHY AND TYPE-II COLLAGEN IMMUNOSTAINING OF THE MANDIBULAR CONDYLE FROM WEANLING RATS [J].
LUDER, HU ;
LEBLOND, CP ;
VONDERMARK, K .
AMERICAN JOURNAL OF ANATOMY, 1988, 182 (03) :197-214
[19]   Mammalian cell cycle checkpoints: signalling pathways and their organization in space and time [J].
Lukas, J ;
Lukas, C ;
Bartek, J .
DNA REPAIR, 2004, 3 (8-9) :997-1007
[20]   Identification of estrogenic compounds in wastewater effluent [J].
Nakada, N ;
Nyunoya, H ;
Nakamura, M ;
Hara, A ;
Iguchi, T ;
Takada, H .
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2004, 23 (12) :2807-2815