Stopped-flow kinetic fluorimetric studies of the interaction of Ru(II) complex with DNA and its analytical application

被引：5

作者：

Wang, Rui-Yong ^{[1
]}

Ji, Mi-Na ^{[2
]}

Wang, Ruiqiang ^{[2
]}

Shi, Jie ^{[1
]}

机构：

[1] Zhengzhou Univ, Dept Chem, Zhengzhou 450001, Peoples R China

[2] Zhengzhou Univ, Sch Nursing, Zhengzhou 450052, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2008年 / 71卷 / 03期

关键词：

Stopped-flow; Fluorimetric; DNA; Ru(phen)(2)(dppz)(2+); Kinetics;

D O I：

10.1016/j.saa.2008.02.043

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

A simple, rapid, and sensitive stopped-now kinetic fluorometric approach was established for the assay of DNA in synthetic samples and real samples by using the measures of initial reaction rate. The increased initial reaction rate is in proportion to the concentration of DNA in the range of 2.0 x 10(-8) M to 2.1 x 10(-6) M. The optimum conditions for various parameters on which the binding of Ru(phen)(2)(dppz)(2+) to DNA depends, were investigated. The influence of various surfactants on the interaction was discussed. Furthermore, stopped-flow techniques were employed to determine kinetic parameters of Ru(phen)(2)(dppZ)(2+) binding to DNA under pseudo-first-order conditions. It was found that the interaction of Ru(phen)(2)(dppZ)(2+) with DNA was very fast. A two-step reaction mechanism, a fast phase followed by a slow phase, was proposed. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1042 / 1048

页数：7

共 50 条

[1] A surfactant enhanced stopped-flow kinetic fluorimetric method for the determination of trace DNA
Wang, RY
Gao, X
Lu, YT
ANALYTICA CHIMICA ACTA, 2005, 538 (1-2) : 151 - 158
[2] Is it possible to study the kinetic parameters of interaction between PNA and parallel and antiparallel DNA by stopped-flow fluorescence?
Barbero, N.
Cauteruccio, S.
Thakare, P.
Licandro, E.
Viscardi, G.
Visentin, S.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2016, 163 : 296 - 302
[3] Synthesis, Biophysical Interaction of DNA/BSA, Equilibrium and Stopped-Flow Kinetic Studies, and Biological Evaluation of bis(2-Picolyl)amine-Based Nickel(II) Complex
Ramzy, Esraa
Ibrahim, Mohamed M.
El-Mehasseb, Ibrahim M.
Ramadan, Abd El-Motaleb M.
Elshami, Fawzia I.
Shaban, Shaban Y.
van Eldik, Rudi
BIOMIMETICS, 2022, 7 (04)
[4] Stopped-flow kinetic method for the fluorimetric determination of DNA traces in biological samples based on the interaction long-wavelength fluorophor, surfactant and nucleic acid
Manzano-Ayala, H. R.
Romero, J. M. Fernandez
Gomez-Hens, A.
ANALYTICA CHIMICA ACTA, 2009, 632 (01) : 109 - 114
[5] STOPPED-FLOW SPECTROPHOTOMETRIC STUDIES OF THE KINETICS OF INTERACTION OF DIHYDROXYFUMARIC ACID WITH THE DPPH FREE RADICAL
Secara, Natalia
CHEMISTRY JOURNAL OF MOLDOVA, 2010, 5 (02): : 83 - 87
[6] Stopped-Flow Fluorescence Kinetic Study of Protein Sliding and Intersegment Transfer in the Target DNA Search Process
Esadze, Alexandre
Iwahara, Junji
JOURNAL OF MOLECULAR BIOLOGY, 2014, 426 (01) : 230 - 244
[7] Voltammetric study of the interaction of lomefloxacin (LMF)-Mg(II) complex with DNA and its analytical application
Zhang, N
Zhang, XL
Zhao, YF
MICROCHEMICAL JOURNAL, 2003, 75 (03) : 249 - 254
[8] Stopped-flow kinetic determination of thiocyanates and thiosulphates with the application of iodine-azide reaction
Kurzawa, J
Janowicz, K
Suszka, A
ANALYTICA CHIMICA ACTA, 2001, 431 (01) : 149 - 155
[9] DNA Binding and Cleavage, Stopped-Flow Kinetic, Mechanistic, and Molecular Docking Studies of Cationic Ruthenium(II) Nitrosyl Complexes Containing "NS4" Core
Shereef, Hadeer A.
Moemen, Yasmine S.
Elshami, Fawzia I.
El-Nahas, Ahmed M.
Shaban, Shaban Y.
van Eldik, Rudi
MOLECULES, 2023, 28 (07):
[10] Stopped-flow kinetic studies of sphere-to-rod transitions of sodium alkyl sulfate micelles induced by hydrotropic salt
Zhang, Jingyan
Ge, Zhishen
Jiang, Xiaoze
Hassan, P. A.
Liu, Shiyong
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2007, 316 (02) : 796 - 802

← 1 2 3 4 5 →