Electrochemical detection of Salmonella using gold nanoparticles

被引：115

作者：

Afonso, Andre S. ^{[1
,2
,3
]}

Perez-Lopez, Briza ^{[1
,4
]}

Faria, Ronaldo C. ^{[2
]}

Mattoso, Luiz H. C. ^{[3
]}

Hernandez-Herrero, Manuela ^{[5
]}

Xavier Roig-Sagues, Artur ^{[5
]}

Maltez-da Costa, Marisa ^{[1
]}

Merkoci, Arben ^{[1
,6
]}

机构：

[1] Univ Autonoma Barcelona, Nanobioelect & Biosensors Grp, Catalan Inst Nanotechnol, CIN2 ICN CSIC, Bellaterra 08193, Catalonia, Spain

[2] Univ Fed Sao Carlos, Dept Quim, BR-13565905 Sao Paulo, Brazil

[3] Embrapa Instrumentacao Agropecuaria, Lab Nacl Nanotecnol Agronegocio, BR-13560970 Sao Paulo, Brazil

[4] LEITAT Technol Ctr, Terrassa 08225, Spain

[5] Univ Autonoma Barcelona, CERPTA, XaRTA, TECNIO,Dept Ciencia Anim & Aliments,Fac Vet, E-08193 Barcelona, Spain

[6] ICREA, Barcelona, Spain

来源：

BIOSENSORS & BIOELECTRONICS | 2013年 / 40卷 / 01期

关键词：

Gold nanoparticles; Magneto-immunoassay; Salmonella; Electrochemical detection; Label; DNA; BACTERIA;

D O I：

10.1016/j.bios.2012.06.054

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A disposable immunosensor for Salmonella enterica subsp. enterica serovar Typhimurium LT2 (S) detection using a magneto-immunoassay and gold nanoparticles (AuNPs) as label for electrochemical detection is developed. The immunosensor is based on the use of a screen-printed carbon electrode (SPCE) that incorporates a permanent magnet underneath. Salmonella containing samples (i.e. skimmed milk) have been tested by using anti-Salmonella magnetic beads (MBs-pSAb) as capture phase and sandwiching afterwards with AuNPs modified antibodies (sSAb-AuNPs) detected using differential pulse voltammetry (DPV). A detection limit of 143 cells mL(-1) and a linear range from 10(3) to 10(6) cells mL(-1) of Salmonella was obtained, with a coefficient of variation of about 2.4%. Recoveries of the sensor by spiking skimmed milk with different quantities of Salmonella of about 83% and 94% for 1.5 x 10(3) and 1.5 x 10(5) cells mL(-1) were obtained, respectively. This AuNPs detection technology combined with magnetic field application reports a limit of detection lower than the conventional commercial method carried out for comparison purposes in skimmed milk samples (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：121 / 126

页数：6

共 24 条

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